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Mouse CD45.1+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse CD45.1 antibody and then magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45.1+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Product Details
- Kit Contents
For Cat. No. 480117:
- 1 vial containing 200 µl of Biotin anti-mouse CD45.1 antibody (clone A20)
- 1 vial containing 200 µl Streptavidin Nanobeads
For Cat. No. 480118:
- 2 vials containing 1 ml each of Biotin anti-mouse CD45.1 antibody (clone A20)
- 2 vials containing 1 ml each of Streptavidin Nanobeads
- Antibody Type
Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
The antibodies were purified by affinity chromatography, and conjugated wtih biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
Volume of Streptavidin Nanobeads should be adjusted depending on the starting percentage of CD45.1+ cells to be isolated. Use the table below as an example when working with a mixture of SJL (CD45.1+) and C57BL/6 (CD45.2+) mouse splenocytes. For 1x107 cells in 100 µl of buffer, use the following volumes:
SJL + C57BL/6 Mixture (spleen)
Approximate Starting CD45.1 Frequency
Optimal Nanobeads Volume
10% SJL + 90% C57BL/6
25% SJL + 75% C57BL/6
50% SJL + 50% C57BL/6
75% SJL + 25% C57BL/6
The volumes indicated in the table are for the use of MojoSort™ magnet (Cat. Nos. 480019/480020). For low frequency cells, pre-dilute the Streptavidin Nanobeads in order to pipette a minimum of 5 µl of any solution. For example, to isolate CD45.1+ cells from a mixture of 25% SJL and 75% C57BL/6, pre-dilute 10 µl of Streptavidin Nanobeads in 40 µl of MojoSort™ buffer (Cat. No. 480017) and add 10 µl of that dilution per sample. Avoid working with small volumes.
- Application Notes
This kit is designed for the positive selection or depletion of mouse CD45.1+ cells from lymphoid tissue.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 15X*
Nanobead dilution to use in columns: 4X*
*Note: Suggested dilutions for column use above determined based on a starting cell population consisting of a 1:1 mix of CD45.1+ and CD45.2+ cells. Further optimization may be necessary for different degrees of heterogeneity in starting cell populations.
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Gene ID
- View information about CD45.1 on UniProt.org
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
The average diameter is approximately 130 nm.