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A single cell suspension comprised of 30% human peripheral blood mononuclear cells (PBMCs) and 70% human colorectal adenocarcinoma cell line (HT-29) cells were prepared to isolate CD45+ cells using the MojoSort™ Human CD45 Nanobeads. Cells were stained with a CD3/CD19/CD56/CD14/CD15 PE cocktail. Dead cells were excluded by 7-AAD. -
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480029 | 20 tests | £103.00 | ||||
| 480030 | 200 tests | £383.00 | ||||
CD45+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD45 nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
-
For Cat# 480029:
- 1 vial containing 200 µl of CD45 nanobeads
For Cat# 480030:
- 2 vials containing 1 ml each of CD45 Nanobeads
Product Details
- Reactivity
- Human
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Antibody-conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Human CD45 Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl Human CD45 Nanobeads for 1x107 cells in 100 µl of buffer.
- Application Notes
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This kit is designed for the positive selection or depletion of CD45+ cells from lymphoid or tissues.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X - Product Citations
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Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Gene ID
- NA
- UniProt
- View information about CD45 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
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Please contact our technical service team for further assistance.
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