LEGENDplex™ Buffer Set B

Pricing & Availability
Regulatory Status
RUO
Other Names
Multiplex assay, Flow cytometry
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price  
740373 100 tests £68.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote

To purchase, view our LEGENDplex™ Mix and Match page

Description

LEGENDplex™ Buffer Set B includes the following items:

  • Setup Beads
  • SA-PE
  • Assay Buffer
  • Wash Buffer
  • Plate Sealers
  • Matrix C

This Buffer Set does not include plates or the Capture Beads, Standard, and Detection Antibodies needed to complete a LEGENDplex™ Assay.

These additional components can be purchased separately by selecting the Mix & Match tab at
www.biolegend.com/legendplex, and then navigating to your desired assay.

LEGENDplex™ Buffer Set B is designed for use with the following LEGENDplex™ Mix and Match Panels:

  • Mouse Cytokine Panel 2
  • Mouse Inflammation Panel
  • Rat Inflammation Panel
  • Rat Th Cytokine Panel
  • Mouse Macrophage/Microglia Panel
  • Mouse B Cell Panels
Product Details
Technical Data Sheet (pdf)

Product Details

Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Product Citations
  1. Meek SM, et al. 2018. Pathogens. 7:22. PubMed
  2. Aguayo–Mazzucato C, et al. 2019. Cell Metab. 30:129. PubMed

Antigen Details

Gene ID
NA

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 5    Revision Date: 05/13/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account