- Regulatory Status
- Other Names
- ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13
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- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|441907||1 Pre-coated Plate||£270.00|
CXCL13, also known as B-Lymphocyte Chemoattractant (BLC), or B Cell-Attracting chemokine 1 (BCA-1), is a small cytokine belonging to the CXC chemokine family. As a strong chemoattractant for B cells, it is highly expressed in the follicles of Peyer’s patches, spleen, and lymph nodes. It is also expressed by T follicular helper cells, dendritic cells, and stromal cells (including marginal reticular cells (MRCs) and follicular dendritic cells (FDCs)) in B cell areas of secondary lymphoid tissue. CXCL13 and its receptor CXCR5 regulate compartmentalization of B- and T-cells in secondary lymphoid organs. During the migration in the follicle, B cells are in contact with FDCs, MRCs, and cells localized around the follicle perimeter, such as sinus-associated macrophages and T cell zone associated DCs. Subsequent to the migration through the FDC network, B cells travel through the T cell zone-proximal follicle. In this migration, CCL21, CCL19, CCR7 and CXCR5 are involved. CXCL13, CCL19, and CCL21 take part in the formation of tertiary lymphoid tissues in chronic antigen-induced arthritis. In CXCL13 deficient mice, B-cells fail to organize in polarized follicular clusters and instead appeare as a ring of cells at the perimeter of T-cell areas.
CXCL13 is highly expressed at sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions. High expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, rheumatoid arthritis, and the allergic airway inflammatory process.
LEGEND MAX™ Mouse CXCL13 (BLC) ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse CXCL13 antibody. The detection antibody is a biotinylated rat monoclonal anti-mouse CXCL13 antibody. This kit is specifically designed for the accurate quantitation of mouse CXCL13 from cell culture supernatant, serum and plasma. This kit is analytically validated with ready-to-use reagents.
- Kit Contents
- Anti-Mouse CXCL13 Precoated 96-well Strip Microplate
- Mouse CXCL13 Detection Antibody
- Mouse CXCL13 Standard
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Additional Product Notes
The 5 pre-coated plate format of this assay will be discontinued on December 31st, 2018. The assay will remain available for purchase under the 1 pre-coated plate catalog number. Starting in 2019, orders with quantities = 5 of the 1 plate kit will have an automatic 30% discount applied to the kit price at checkout.
- 1.0 ± 0.4 pg/mL
- Standard Range
- 7.8 - 500 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.