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LEGEND MAX™ Human FGL2 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
FGL2 Pre-coated ELISA Kit
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Product Citations
publications
Human_IL-22_112910
  • Human_IL-22_112910
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436907 1 Pre-coated Plate £317
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Description

Fibrinogen-like protein 2 (FGL2), also known as fibroleukin, was first cloned from cytotoxic T lymphocytes and was classified as a member of the fibrinogen superfamily due to its homology (36%) with fibrinogen b and g chains. In humans, the fgl2 gene encodes a protein of 439 amino acids. Under non-reducing conditions, the molecular mass of the protein is 250-300 kD and in reducing conditions, it is 64-70 kD, indicating that FGL2 in its natural state forms a tetrameric complex. It is postulated that FGL2 has pleiotropic effects and is an important immune regulator of both innate and adaptive responses. In macrophages and endothelial cells, FGL2 is expressed as a membrane-associated protein, which acts as a prothrombinase enzyme with the ability to generate thrombin directly from prothrombin. FGL2 also exists as a secreted form expressed by regulatory T cells that down-regulates dendritic cell activity and induces B cell apoptosis. The regulatory activity of FGL2 has been implicated in inhibition of allograft rejection, autoimmunity, and the pathogenesis of experimental as well as human viral infections.

BioLegend's Human FGL2 LEGEND MAX™ ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with 96 well microtiter strip plates that are pre-coated with a capture antibody.

This Human FGL2 ELISA Kit with pre-coated plates is specifically designed for the accurate quantitation of analytes from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated, with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human FGL2 Pre-coated 96-well Strip Microplate
  • Human FGL2 Dectection Antibody
  • Human FGL2 Standard
  • Matrix C (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA
Product Citations
  1. You Y, et al. 2021. Mol Med Rep. 24:. PubMed
  2. El-Mesery M, et al. 2021. J Interferon Cytokine Res. 41:52. PubMed
  3. Lei J, et al. 2021. Theranostics. 4251:11. PubMed
Sensitivity
45 pg/mL
Standard Range
0.25-16 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Tregs that down-regulates dendritic cell activity and induces B cell apoptosis
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
10875 View all products for this Gene ID
UniProt
View information about FGL2 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 3    Revision Date: 02/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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