LEAF™ Purified anti-mouse CD1d Antibody

Pricing & Availability
Clone
K253 (See other available formats)
Other Names
Antigen-presenting glycoprotein CD1d1, CD1.1, Ly-38
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
K253_LEAF_CD1d_Antibody_FC_052413
C57BL/6 mouse splenocytes were stained purified CD1d (clone K253, filled histogram) or mouse IgG1 isotype control, followed by anti-mouse IgG1 PE.
  • K253_LEAF_CD1d_Antibody_FC_052413
    C57BL/6 mouse splenocytes were stained purified CD1d (clone K253, filled histogram) or mouse IgG1 isotype control, followed by anti-mouse IgG1 PE.
Cat # Size Price Quantity Avail. Save
140804 500 µg £188
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This product is scheduled for discontinuation as a part of our ongoing LEAF™ to Ultra-LEAF™ antibody transition. The equivalent Ultra-LEAF™ product may be available at comparable or significantly lower price. Please use the availability checker to determine current availability, or contact technical service for further inquiries.
Description

CD1d is a type I transmembrane protein and member of the MHC family, with a molecular weight ranging from 43-49 kD, depending on the glycosylation degree. CD1d is expressed by antigen presenting cells such as dendritic cells, monocytes, macrophages and B cells; also expressed by thymocytes and intestinal epithelial cells. CD1d present glycolipids to iNKT cells that recognize them by their Vα14i TCR.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
α-GalCer:mCD1d complexes coupled to Mycobacterium tuberculosis PPD
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA1, immunofluorescence2 to detect CD1d in the lipid rafts on the cell membrane, and block1 CD1d recognition of an iNKT cell hybridoma.

Application References

(PubMed link indicates BioLegend citation)
  1. Yu KO, et al. 2007. J. Immunol. Methods 323:11. (ELISA, Block)
  2. Im JS, et al. 2009. Immunity 30:888. (IF)
Product Citations
  1. Yuan C, et al. 2018. iScience. 11:13. PubMed
RRID
AB_10639732 (BioLegend Cat. No. 140804)

Antigen Details

Structure
Member of the MHC family. Type I transmembrane protein. Associates with β2-microglobulin. Molecular weight ranges from 43 - 49 kD, depending on glycosylation.
Distribution

Dendritic cells, monocytes, macrophages, B cells, thymocytes, intestinal epithelial cells

Function
Glycolipid presentation to iNKT cells
Ligand/Receptor
Vα14i TCR
Cell Type
B cells, Dendritic cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.

Gene ID
12479 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 06/04/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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