LEAF™ Purified anti-mouse CD18 Antibody

Pricing & Availability
Clone
M18/2 (See other available formats)
Other Names
integrin β2, β2 integrin, ITGB2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M18slash2_LEAF_080207
C57BL/6 mouse splenocytes stained with LEAF™ purified M18/2, followed by anti-rat IgG FITC
  • M18slash2_LEAF_080207
    C57BL/6 mouse splenocytes stained with LEAF™ purified M18/2, followed by anti-rat IgG FITC
Cat # Size Price Quantity Avail. Save
101409 50 µg £50
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101410 500 µg £168
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Description

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL glycoprotein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, WB, Block, Stim, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101410).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
  2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
  3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
  4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
  5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
  6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
  7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)
RRID
AB_312818 (BioLegend Cat. No. 101409)
AB_2128343 (BioLegend Cat. No. 101410)

Antigen Details

Structure
Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution
All leukocytes
Function
Adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Gene ID
16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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