Keratin 14 Polyclonal Antibody, Purified

Pricing & Availability
Clone
Poly19053 (See other available formats)
Other Names
Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
Previously
Covance Catalog# PRB-155P
Isotype
Rabbit Polyclonal
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Product Citations
publications
KifLiakath
Whole mount mouse tail epidermis was stained with purified anti-Keratin 14 (green, clone Poly19053), purified anti-Keratin 15 (orange, clone Poly18339) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Kif Liakath.
  • KifLiakath
    Whole mount mouse tail epidermis was stained with purified anti-Keratin 14 (green, clone Poly19053), purified anti-Keratin 15 (orange, clone Poly18339) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Kif Liakath.
  • Poly19053_Purified_Keratin_14_Antibody_IHC_030615
    Strong cytoplasmic staining of the epidermis in normal human skin tissue detected with anti-K14 (clone Poly19053).
Cat # Size Price Quantity Avail. Save
905301 100 µl £251
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19053 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 14. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Reactivity
Human, Mouse, Rat, Dog, Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by peptide affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored between 2°C and 8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues, frozen tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wend P, et al. Wnt/?-catenin signalling induces MLL to create epigenetic changes in salivary gland tumours. EMBO J., Jun 2013.
  2. Welm AL, Kim S, Welm BE, Bishop JM. MET and MYC cooperate in mammary tumorigenesis. Proc Natl Acad Sci USA 102(12):4324-9, 2005. [IHC] PubMed
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
  6. Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
  7. Easter SL, et al. 2014. PLoS One. 9:113247. (IF, WB) PubMed
  8. Frew IJ, et al. 2008. Mol Cell Biol.28:4536. (IHC) PubMed
  9. Levy V, et al. 2007. FASEB J. 21:1358. (IHC) PubMed
  10. Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
  11. Smith BA, et al. 2012. Genes Cancer.3:550. (IHC) PubMed
Product Citations
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  2. Welm A, et al. 2005. Proc Natl Acad Sci U S A. 102:4324-4329. PubMed
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  4. Frew I, et al. 2008. Mol Cell Biol. 28:4536-4548. PubMed
  5. Levy V, et al. 2007. FASEB J. 21:1358-1366. PubMed
  6. Sengupta A, et al. 2010. PLoS One. 5:e12249. PubMed
  7. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  8. Easter S, et al. 2014. PLoS One. 9:e113247. PubMed
  9. Cheung K, et al. 2016. Proc Natl Acad Sci U S A. 113: 854 - 863. PubMed
  10. Lichtenberger B, Watt M 2016. Nat Commun. 7:10537. PubMed
  11. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
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  16. Panousopoulou E, et al. 2016. J Cell Sci. 129: 1915 - 1927. PubMed
  17. Sada A, et al. 2016. Nat Cell Biol. 18: 619-631. PubMed
  18. Annunziato S, et al. 2016. Genes Dev. 30: 1470 - 1480. PubMed
  19. Wuidart A, et al. 2016. Genes Dev. 30: 1261 - 1277. PubMed
  20. Nahidiazar L, et al. 2016. PLoS One. 11: 0158884. PubMed
  21. E Joo, I Lombaert, K Yamada 2016. J Dent Res. 95(13):1518-1527. PubMed
  22. Hasche D, et al. 2016. PLoS One. 11: 0161283. PubMed
  23. Yoo K, et al. 2016. Mol Cell Biol. 36: 2108 - 2120. PubMed
  24. Goguet-Rubio P, et al. 2016. Proc Natl Acad Sci U S A. 113: 11004 - 11009. PubMed
  25. Shatirishvili M, et al. 2016. Cell Death Dis. 7:e2461. PubMed
  26. Feinberg T, et al. 2016. Development. 143: 3956 - 3968. PubMed
  27. Goreczny G, et al. 2016. Oncogene. 10.1038/onc.2016.422. PubMed
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  29. Guorui H, et al. 2017. Nat Commun. 8:14351. PubMed
  30. Tao L, et al. 2017. Nat Commun. 8:14431. PubMed
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  37. Price AJ, et al. 2018. Nat Commun. 9:5284. PubMed
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  39. Li L, et al. 2018. J Clin Invest. 128:4525. PubMed
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  47. Gao Y, et al. 2019. Life Sci Alliance. 2:3. PubMed
  48. Devos M, et al. 2017. J Invest Dermatol. 137:494. PubMed
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  50. Sirka OK, et al. 2018. J Cell Biol. 217:3368. PubMed
  51. Smith P, et al. 2019. Sci Rep. 9:7079. PubMed
  52. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
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Publication Library
RRID
AB_2565048 (BioLegend Cat. No. 905301)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3861 View all products for this Gene ID 3861 View all products for this Gene ID
UniProt
View information about Keratin 14 on UniProt.org

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.

Go To Top Version: 4    Revision Date: 05/11/2017

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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