Keratin 14 Polyclonal Antibody, Purified

Pricing & Availability
Poly19053 (See other available formats)
Other Names
Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
Covance Catalog# PRB-155P
Rabbit Polyclonal
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Product Citations
Whole mount mouse tail epidermis was stained with purified anti-Keratin 14 (green, clone Poly19053), purified anti-Keratin 15 (orange, clone Poly18339) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Kif Liakath.
  • KifLiakath
    Whole mount mouse tail epidermis was stained with purified anti-Keratin 14 (green, clone Poly19053), purified anti-Keratin 15 (orange, clone Poly18339) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Kif Liakath.
  • Poly19053_Purified_Keratin_14_Antibody_IHC_030615
    Strong cytoplasmic staining of the epidermis in normal human skin tissue detected with anti-K14 (clone Poly19053).
Cat # Size Price Quantity Avail. Save
905301 100 µl £251
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19053 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 14. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Human, Mouse, Rat, Dog, Primate
Antibody Type
Host Species
Phosphate-buffered solution + 0.03% Thimerosal.
The antibody was purified by peptide affinity chromatography.
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored between 2°C and 8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.


Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues, frozen tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wend P, et al. Wnt/?-catenin signalling induces MLL to create epigenetic changes in salivary gland tumours. EMBO J., Jun 2013.
  2. Welm AL, Kim S, Welm BE, Bishop JM. MET and MYC cooperate in mammary tumorigenesis. Proc Natl Acad Sci USA 102(12):4324-9, 2005. [IHC] PubMed
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
  6. Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
  7. Easter SL, et al. 2014. PLoS One. 9:113247. (IF, WB) PubMed
  8. Frew IJ, et al. 2008. Mol Cell Biol.28:4536. (IHC) PubMed
  9. Levy V, et al. 2007. FASEB J. 21:1358. (IHC) PubMed
  10. Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
  11. Smith BA, et al. 2012. Genes Cancer.3:550. (IHC) PubMed
Product Citations
  1. Böttcher J, et al. 2015. J Immunol. 195: 5066 - 5076. PubMed
  2. Welm A, et al. 2005. Proc Natl Acad Sci U S A. 102:4324-4329. PubMed
  3. Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
  4. Frew I, et al. 2008. Mol Cell Biol. 28:4536-4548. PubMed
  5. Levy V, et al. 2007. FASEB J. 21:1358-1366. PubMed
  6. Sengupta A, et al. 2010. PLoS One. 5:e12249. PubMed
  7. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  8. Easter S, et al. 2014. PLoS One. 9:e113247. PubMed
  9. Cheung K, et al. 2016. Proc Natl Acad Sci U S A. 113: 854 - 863. PubMed
  10. Lichtenberger B, Watt M 2016. Nat Commun. 7:10537. PubMed
  11. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
  12. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed
  13. Dahlhoff M, et al. 2016. Development. 143: 1823 - 1831. PubMed
  14. Kriegbaum M, et al. 2016. Sci Rep. 6: 25833. PubMed
  15. Turpin J, et al. 2016. Oncogene. 10.1038/onc.2016.129. PubMed
  16. Panousopoulou E, et al. 2016. J Cell Sci. 129: 1915 - 1927. PubMed
  17. Sada A, et al. 2016. Nat Cell Biol. 18: 619-631. PubMed
  18. Annunziato S, et al. 2016. Genes Dev. 30: 1470 - 1480. PubMed
  19. Wuidart A, et al. 2016. Genes Dev. 30: 1261 - 1277. PubMed
  20. Nahidiazar L, et al. 2016. PLoS One. 11: 0158884. PubMed
  21. E Joo, I Lombaert, K Yamada 2016. J Dent Res. 95(13):1518-1527. PubMed
  22. Hasche D, et al. 2016. PLoS One. 11: 0161283. PubMed
  23. Yoo K, et al. 2016. Mol Cell Biol. 36: 2108 - 2120. PubMed
  24. Goguet-Rubio P, et al. 2016. Proc Natl Acad Sci U S A. 113: 11004 - 11009. PubMed
  25. Shatirishvili M, et al. 2016. Cell Death Dis. 7:e2461. PubMed
  26. Feinberg T, et al. 2016. Development. 143: 3956 - 3968. PubMed
  27. Goreczny G, et al. 2016. Oncogene. 10.1038/onc.2016.422. PubMed
  28. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  29. Guorui H, et al. 2017. Nat Commun. 8:14351. PubMed
  30. Tao L, et al. 2017. Nat Commun. 8:14431. PubMed
  31. Larribere L,et al. 2017. Stem Cell Reports.. 10.1016/j.stemcr.2017.08.023. PubMed
  32. Dravis C 2018. Cancer cell. 34:466. PubMed
  33. Trisno SL 2018. Cell stem cell. 23:501. PubMed
  34. Wang J, et al. 2017. Cancer Prev Res (Phila). 10:684. PubMed
  35. Reeves MQ, et al. 2018. Nat Cell Biol. 20:699. PubMed
  36. Roux LN, et al. 2018. Stem Cells. 36:1421. PubMed
  37. Price AJ, et al. 2018. Nat Commun. 9:5284. PubMed
  38. Sachs N, et al. 2019. EMBO J. 38:. PubMed
  39. Li L, et al. 2018. J Clin Invest. 128:4525. PubMed
  40. Sundberg JP, et al. 2018. PLoS One. 13:e0205775. PubMed
  41. Santoro A, et al. 2019. Cell Rep. 26:624. PubMed
  42. Choo MK, et al. 2018. Sci Signal. 11:551. PubMed
  43. Flores A, et al. 2019. Nat Commun. 10:91. PubMed
  44. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  45. Han Y, et al. 2019. Cell Rep. 27:835. PubMed
  46. Ren G, et al. 2019. Sci Rep. 9:6688. PubMed
  47. Gao Y, et al. 2019. Life Sci Alliance. 2:3. PubMed
  48. Devos M, et al. 2017. J Invest Dermatol. 137:494. PubMed
  49. Yamamoto–Fukuda T, et al. 2018. J Assoc Res Otolaryngol. 19:223. PubMed
  50. Sirka OK, et al. 2018. J Cell Biol. 217:3368. PubMed
  51. Smith P, et al. 2019. Sci Rep. 9:7079. PubMed
  52. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  53. Norum JH, et al. 2019. Int J Cancer. N/A. PubMed
  54. Castillo–Azofeifa D, et al. 2018. Development. 145. PubMed
  55. May AJ, et al. 2018. Development. 145. PubMed
  56. Stratikopoulos EE, et al. 2019. Oncogene. 38:47. PubMed
  57. Kim SJ, et al. 2019. Oncogene. 38:5942. PubMed
  58. Schipper K, et al. 2019. Nat Commun. 10:3800. PubMed
  59. Zhao C, et al. 2017. Science. 356. PubMed
Publication Library
AB_2565048 (BioLegend Cat. No. 905301)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3861 View all products for this Gene ID 3861 View all products for this Gene ID
View information about Keratin 14 on

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Go To Top Version: 4    Revision Date: 05/11/2017

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