Keratin 10 Polyclonal Antibody, Purified

Pricing & Availability
Clone
Poly19054 (See other available formats)
Isotype
Rabbit Polyclonal
Purified_Keratin-10_Antibody_IHC_021715
Strong cytoplasmic staining of the epidermis in normal human skin tissue using K10 (clone Poly19054).
  • Purified_Keratin-10_Antibody_IHC_021715
    Strong cytoplasmic staining of the epidermis in normal human skin tissue using K10 (clone Poly19054).
Cat # Size Price Quantity Avail. Save
905401 100 µL £256
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Instructions for Use (pdf)

Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19054 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 10. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity
Mouse, Human, Dog, Primate
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 10 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by peptide affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams JR, Xu K, Liu JC, Ruiz Agamez NM, Loch AJ, Wong RG, Wang W, Wright KL, Lane TF, Zacksenhaus E, and Egan SE. Cooperation between Pik3ca and p53 Mutations in Mouse Mammary Tumor Formation. Cancer Res., 71: 2706-2717, Apr 2011.
  2. Lacroix M, et al. Transcription factor E4F1 is essential for epidermal stem cell maintenance and skin homeostasis. PNAS, 107: 21076-21081, Dec 2010. [IHC] PubMed
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
  6. Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
  7. Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and PTrez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. [IHC] PubMed
  8. DiTommaso T, et al. 2014. PLoS Genet. 10:1004706. (IHC) PubMed
Product Citations
  1. Lacroix M, et al. 2010. Proc Natl Acad Sci U S A. 107:21076-21081. PubMed
  2. Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
  3. Bayo P, et al. 2008. Endocrinology. 149:1377-1388. PubMed
  4. DiTommaso T, et al. 2014. PLoS Genet. 10:e1004706. PubMed
  5. Wu N, et al. 2016. J Invest Dermatol. 136:192-201. PubMed
  6. Li Z, et al. 2016. Nat Commun. 7: 11394. PubMed
  7. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed
  8. Dahlhoff M, et al. 2016. Development. 143: 1823 - 1831. PubMed
  9. Kriegbaum M, et al. 2016. Sci Rep. 6: 25833. PubMed
  10. Blanco S, et al. 2015. Nature. 534:335-340. PubMed
  11. Goguet-Rubio P, et al. 2016. Proc Natl Acad Sci U S A. 113: 11004 - 11009. PubMed
  12. Callejas-Valera J, et al. 2016. Carcinogenesis. 37: 1014 - 1025. PubMed
  13. Shatirishvili M, et al. 2016. Cell Death Dis. 7:e2461. PubMed
  14. Lin J, et al. 2016. Nature. 540:124-128. PubMed
  15. Mathew G, et al. 2016. Proc Natl Acad Sci U S A. 113: 13156 - 13161. PubMed
  16. Madon-Simon M, et al. 2017. PLoS One. 12(6):e0180035. PubMed
  17. Rogerson C, et al. 2018. Biochim Biophys Acta. 37:2022. PubMed
  18. Michel C, et al. 2017. J Immunol. 199:3488. PubMed
  19. Yang CS, et al. 2017. Prostate. 77:1452. PubMed
  20. Murphy SV, et al. 2017. Stem Cells Transl Med. 6:2020. PubMed
  21. Devos M, et al. 2017. J Invest Dermatol. 137:494. PubMed
  22. Yamamoto–Fukuda T, et al. 2018. J Assoc Res Otolaryngol. 19:223. PubMed
  23. Eichelberg MR, et al. 2019. MBio. 10. PubMed
  24. Sajini AA, et al. 2019. Nat Commun. 10:2550. PubMed
  25. Ehrlich F, et al. 2019. Sci Rep. 9:10924. PubMed
  26. Laurin M, et al. 2019. Elife. 8. PubMed
  27. Song Y et al. 2018. Cell reports. 25(11):2981-2991 . PubMed
  28. Hegde GV et al. 2019. Elife. 8 pii: e46551. PubMed
  29. Köhler C et al. 2017. Cell stem cell. 21(5):679-693 . PubMed
RRID
AB_2565049 (BioLegend Cat. No. 905401)

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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