Keratin 1 Polyclonal Antibody, Purified

Pricing & Availability
Clone
Poly19052 (See other available formats)
Isotype
Rabbit Polyclonal
Purified_Keratin_Antibody_IHC_021615
Immunohistochemistry with anti-K1 (Cat: 905201) showing strong cytoplasmic staining of the epidermis in normal human skin tissue.
  • Purified_Keratin_Antibody_IHC_021615
    Immunohistochemistry with anti-K1 (Cat: 905201) showing strong cytoplasmic staining of the epidermis in normal human skin tissue.
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905201 100 µL £256
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Instructions for Use (pdf)

Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19052 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 1. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity
Human, Rat, Primate
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 1 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by peptide affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:500 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu N, Rollin J, Masse I, Lamartine J, and Gidrol X. p63 Regulates Human Keratinocyte Proliferation via MYC-regulated Gene Network and Differentiation Commitment through Cell Adhesion-related Gene Network. J. Biol. Chem.,287: 5627-5638, Feb 2012. PubMed
  2. Schütz M, Auth T, Gehrt A, Bosen F, K÷rber I, Strenzke N, Moser T, and Willecke K. The connexin26 S17F mouse mutant represents a model for the human hereditary keratitisùichthyosisùdeafness syndrome. Hum. Mol. Genet.; 20: 28-39, Jan 2011.
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
Product Citations
  1. Wu N, et al. 2012. J Biol Chem. 287:5627-5638. PubMed
  2. Shigehara Y, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw277. PubMed
  3. Mollinedo P, et al. 2019. Sci Rep. 9:736. PubMed
  4. Tata PR, et al. 2018. Dev Cell. 44:679. PubMed
  5. Derr K, et al. 2019. Tissue Eng Part C Methods. 25(6). PubMed
  6. Tanis SEJ et al. 2018. Cell reports. 25(5):1292-1303 . PubMed
RRID
AB_2565047 (BioLegend Cat. No. 905201)

Related FAQs

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Go To Top Version: 4    Revision Date: 05/11/2017

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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