ELISA MAX™ Standard Set Human TNF-α

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Regulatory Status
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Product Citations
human tnf-a_121609
  • human tnf-a_121609
Cat # Size Price Quantity Check Availability Save
430201 5 plates £154.00
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The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number. Please contact your BioLegend sales representative for bulk pricing quotations.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human TNF-α ELISA MAX™ Capture Antibody (200X)
  • Human TNF-α ELISA MAX™ Detection Antibody (200X)
  • Human TNF-α Standard
  • Avidin-HRP (1000X)

Product Details



As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Davis AM, et al. 2008. J. Immunol. 180:6923. PubMed
  2. Kulkarni O, et al. 2009. J. Pharmacol Exp Ther. 328:371. PubMed
  3. Wang X, et al. 2014. Int J Cardiol. 174:688. PubMed
  4. Sautto GA, et al. 2015. Gut. PubMed
Product Citations
  1. Bikker R, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.06.009. PubMed
  2. Kong B, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0961-z. PubMed
  3. Sadewasser A, et al. 2019. Mol Ther Nucleic Acids. 16:686. PubMed
  4. Khongkhunthian S, et al. 2018. Drug Discov Ther. 11:323. PubMed
  5. Mamriev D, et al. 2020. Cell Death Dis. 0.79375. PubMed
  6. Kulkarni O, et al. 2009. J Pharmacol Exp Ther. 328:371. PubMed
  7. Davis A, et al. 2008. J Immunol. 180:6923. PubMed
  8. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  9. Wang X, et al. 2014. Int J Cardiol. 174:6888. PubMed
  10. Sautto G, et al. 2015. Gut. . PubMed
  11. Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
  12. Jø nsson K, et al. 2017. Nat Commun. 8:14391. PubMed
  13. Bibby J, et al. 2020. Nat Commun. 2.827777778. PubMed
  14. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  15. Hoang QTM, et al. 2021. Front Med (Lausanne). 8:676058. PubMed
  16. Ban T, et al. 2021. Nat Commun. 12:4379. PubMed
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
7124 View all products for this Gene ID
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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