HRP anti-Tau, 419-433 Antibody

Pricing & Availability
Clone
A16097D (See other available formats)
Other Names
Microtubule associated protein tau
Isotype
Rat IgG2a, κ
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Product Citations
publications
A16097D_HRP_Tau419-433_Antibody_1_050718
Western blot of HRP anti-Tau, 419-433 antibody (clone A16097D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A16097D_HRP_Tau419-433_Antibody_1_050718
    Western blot of HRP anti-Tau, 419-433 antibody (clone A16097D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A16097D_HRP_Tau419-433_Antibody_2_110817
    IHC staining of HRP anti-Tau, 419-433 antibody (clone A16097D) on formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with 1 µg/mL of the primary antibody for 1 hour at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
851003 25 µg £84
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851004 100 µg £178
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant C-terminal fragment of human 4R Tau protein encompassing amino acid residues 419-433.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunohistochemistry, a concentration range of 0.2 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with 3R and 4R Tau isoforms, and recognizes endogenous human and mouse Tau in brain lysates by WB. 

RRID
AB_2734632 (BioLegend Cat. No. 851003)
AB_2734633 (BioLegend Cat. No. 851004)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.

Cellular distribution: cytoskeleton, nucleus, plasma membrane and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed.
  2. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed.
  3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau, 419-433 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 10/11/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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