HRP anti-Spectrin β1 Antibody

Pricing & Availability
Clone
N385/21 (See other available formats)
Other Names
SPTB, SPTB1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
N385-21_HRP_Spectrin-B1_Antibody_070219.png
Western blot of HRP anti-Spectrin β1 antibody (clone N385/21). Lanes 1,3: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 4: 15 µg of mouse brain lysate; Lane 5: 40 µg of rat brain lysate. The blots were incubated with 2 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N385-21_HRP_Spectrin-B1_Antibody_070219.png
    Western blot of HRP anti-Spectrin β1 antibody (clone N385/21). Lanes 1,3: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 4: 15 µg of mouse brain lysate; Lane 5: 40 µg of rat brain lysate. The blots were incubated with 2 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Avail. Save
856903 25 µg £67
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856904 100 µg £114
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Description

Spectrin β1 (SPTB1) is one of the several beta-spectrins that constitute the major components of the cytoskeletal network in erythrocyte plasma membrane. It binds another structural protein, band 4.1, to form a complex that anchors to the cytoplasmic face of the plasma membrane via Ankyrin, which in turn binds to protein band 3. Mutations in spectrin have been linked to hereditary defects in the erythrocyte, such as hereditary elliptocytosis. Recent studies show that caspase- and calpain-mediated cleavage of spectrin leads to the production of a series of fragments collectively called spectrin breakdown products (SBDPs). Multiple studies have shown that SBDPs may serve as a biomarker for traumatic brain injury (TBI)4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
SPTB1 is a 2137 amino acid protein with an apparent molecular mass of ~ 246 kD.
Distribution

Tissue Distribution: Brain, bone marrow, epididymis, and heart muscle.
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, and golgi apparatus.

Function
SPTB1 is a major constituent of the cytoskeletal network of the erythrocyte plasma membrane.
Interaction
EPB41 , actin, ankyrin
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Presynaptic proteins, Scaffold Proteins
Antigen References
  1. Zhang R, et al. 2013. Sci China Life Sci. 56(12):1076
  2. Machnicka B, et al. 2014. Biochim Biophys Acta. 1838(2):620
  3. Baines AK. 2009. Biochem Soc Trans. 37(Pt4):796
  4. Kovesdi E. 2010. Acta Neurochir (Wien). 152(1):1
Gene ID
6710 View all products for this Gene ID
UniProt
View information about Spectrin beta1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/31/2019

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