HRP anti-RBFOX1 (A2BP1) Antibody

Pricing & Availability
Clone
A18069B (See other available formats)
Other Names
RBFox1, A2BP1, RNA Binding fox-1 Homolog 1, Hexaribonucleotide Binding Protein 1, FOX1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A18069B_HRP_RBFOX1_Antibody_1_100219
Western blot of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate.The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A18069B_HRP_RBFOX1_Antibody_1_100219
    Western blot of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate.The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A18069B_HRP_RBFOX1_Antibody_2_100219
    Western blot of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 10 µg of rat brain lysate; Lane 3: 10 µg of mouse brain Lysate. The blot was incubated with 0.01 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A18069B_HRP_RBFOX1_Antibody_3_100219
    IHC staining of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18069B_HRP_RBFOX1_Antibody_4_100219
    IHC staining of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
862703 25 µg £81
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862704 100 µg £178
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Description

Rbfox1 is a member of the RNA binding protein family that shares a conservative RNA recognition motif. Rbfox1 has been shown to modulate alternative splicing by binding the consensus RNA sequence motif (U)GCAUG within introns. The interaction between cytoplasmic Rbfox1 and mRNA affects mRNA stability and translation. The binding of Rbfox1 protein to the C-terminus of ataxin-2 may be a contributing factor to the development of spinocerebellar ataxia type 2 .

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, a concentration range of 0.02 - 2.0 µg/ml for human and 0.01 - 0.02 µg/ml for murine is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
RBFOX1 is a 416 amino acid protein with an apparent molecular mass of ~ 45 kD.
Distribution

Tissue Distribution: brain and heart.
Cellular Distribution: nucleus, golgi apparatus, and cytosol.

Function
RBFOX1 is an RNA binding protein that modulates mRNA alternative splicing.
Interaction
RNA, ataxin-2
Cell Type
Neurons, Oligodendrocytes
Biology Area
Neurodegeneration, Protein Synthesis, Transcription Factors
Antigen References
  1. Wang ET, et al. 2016. J. Neurosci. 36(45):11418
  2. Duan W, et al. 2016. Mol Neurobiol. 53(3):1637
  3. Kuroyanagi H, 2009. Cell Mol Life Sci. 66(24):3895
Gene ID
54715 View all products for this Gene ID 268859 View all products for this Gene ID 302920 View all products for this Gene ID
UniProt
View information about RBFOX1 (A2BP1) on UniProt.org

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Go To Top Version: 1    Revision Date: 10/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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