- SMI 71 (See other available formats)
- Other Names
- Endothelial barrier antigen, EBA
- Mouse IgM, κ
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SMI 71 is specific for a rat endothelial protein found in areas with blood-brain or blood-nerve barriers. The antibody does not react with endothelia of periventricular organs or with fenestrated endothelia in peripheral tissues. Specifically, it has been shown that staining with SMI 71 is not observed in blood vessels and sinusoids in the liver, vessels in the heart, adrenal, skeletal muscle, intestine, thymus, lymph nodes, pancreas, thyroid, or skin. However, a patchy reaction was observed on some vessel walls of the spleen and epidermis of the skin3. Reactivity with the antibody develops in newborn rats along with maturation of the blood brain barrier. Reactivity disappears in lesions of experimental allergic encephalomyelitis. Contrary to the belief that astrocytes and perhaps neurons are essential for the establishment of the blood-brain barrier, destruction of neurons (monitored by SMI 311 and anti-MAP2) and of astrocytes (monitored by anti-GFAP and anti-S100B) leads only to transient abolition of SMI 71 reactivity and only transient transendothelial passage of serum albumin.Product Details
- Antibody Type
- Host Species
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Gene ID
- View information about Blood-Brain Barrier on UniProt.org
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