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HRP anti-rabbit IgG Antibody

Pricing & Availability
Clone
6B9G9 (See other available formats)
Regulatory Status
RUO
Other Names
N0034
Isotype
Mouse IgG1, κ
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Product Citations
publications
6B9G9_HRP_IgG_WB_Antibody_020717
Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with anti-β-actin antibody (clone Poly6221, rabbit IgG) (lane 1, 2, 3 and 5) and purified anti-β actin antibody as the negative control (clone 2F1-1, mouse IgG) (lane 4). After a 30 minutes wash, lanes 1 and 4, 2, 3 were probed respectively with 1:5000, 1:10000 and 1:20000 HRP anti-rabbit IgG antibodies (clone 6B9G9), and lane 5 was probed with 1:3000 HRP Donkey anti-rabbit IgG (minimal x-reactivity) antibody (clone Poly4064) as a positive control. Proteins were visualized using chemiluminescence detection system. Lane M contains the molecular weight ladder.
  • 6B9G9_HRP_IgG_WB_Antibody_020717
    Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with anti-β-actin antibody (clone Poly6221, rabbit IgG) (lane 1, 2, 3 and 5) and purified anti-β actin antibody as the negative control (clone 2F1-1, mouse IgG) (lane 4). After a 30 minutes wash, lanes 1 and 4, 2, 3 were probed respectively with 1:5000, 1:10000 and 1:20000 HRP anti-rabbit IgG antibodies (clone 6B9G9), and lane 5 was probed with 1:3000 HRP Donkey anti-rabbit IgG (minimal x-reactivity) antibody (clone Poly4064) as a positive control. Proteins were visualized using chemiluminescence detection system. Lane M contains the molecular weight ladder.
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410406 100 µL £221
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Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rabbit IgG
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Optimal concentration of usage is 1:10,000 – 1:40,000 for WB. The optimal concentration should be determined by titration for each individual assay of interest.

 

Product Citations
  1. Uka R, et al. 2020. Oncogene. 39:4132. PubMed
  2. Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed
RRID
AB_2650737 (BioLegend Cat. No. 410406)

Antigen Details

Structure
IgG antibodies are large molecules of about 150 kD made of four peptide chains. It contains two identical class γ heavy chains of about 50 kD and two identical light chains of about 25 kD.
Distribution

Secreted, B cell surface.

Function
Antibodies are major components of humoral immunity. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
Gene ID
100009097 View all products for this Gene ID
UniProt
View information about IgG on UniProt.org
Go To Top Version: 1    Revision Date: 02/08/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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