HRP anti-NSE Antibody

Pricing & Availability
Clone
NSE-P1 (See other available formats)
Other Names
Gamma-enolase, neural enolase, neuron-specific enolase, 2-phospho-D-glycerate hydrolyase, ENO2, NSE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NSE-P1_PURE_NSE_Antibody_1_062619
Western blot of HRP anti-NSE antibody (clone NSE-P1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.001 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • NSE-P1_PURE_NSE_Antibody_1_062619
    Western blot of HRP anti-NSE antibody (clone NSE-P1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.001 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • NSE-P1_PURE_NSE_Antibody_2_062619
    IHC staining of HRP anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P1_PURE_NSE_Antibody_3_062619
    IHC staining of HRP anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P1_PURE_NSE_Antibody_4_062619
    IHC staining of HRP anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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804909 25 µg £71
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Description

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers. Expression of these isozymes can be cell-type and development-specific. This gene encodes gamma enolase (enolase 2) and is expressed in mature neurons and cells of neuronal origin. The alpha/alpha homodimers are expressed in embryo and in most adult tissues. The alpha/beta heterodimers and the beta/beta homodimers are found in striated muscle, and the alpha/gamma heterodimers and the gamma/gamma homodimers are expressed in neurons. Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids 416-433. It recognizes the sequence LGDEARFAGHNFRNPSVL.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 2.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The reactivity of Alexa Fluor® 647 and Alexa Fluor® 488 anti-NSE antibody was validated in FFPE mouse and rat tissue.

Additional reported applications (for the relevant formats) include: ELISA2 and immunohistochemistry1,3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoo J, et al. 2002. Arch. Pathol. Lab Med. 126(8):979. (IHC-P)
  2. Duncan ME, et al. 1992. J. Immuno. Methods. 151:227 (ELISA)
  3. Chetty R. 2000. Pathology. 32(3):209. (IHC-P)
  4. Murray GI, et al. 1993. J. Clin. Pathol. 46:993. (IHC-P)

Antigen Details

Structure
Gamma enolase is a 434 amino acid protein with a molecular mass of 47 kD.
Distribution

Tissue distribution: Nervous and musculoskeletal systems.
Cellular distribution: Secreted, cytosol, plasma membrane, cytoskeleton, and nucleus.

Function
Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Cell Type
Mature Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Enzymes and Regulators, Phospho-Proteins
Antigen References
  1. Muoio B, et al. 2017. Int J Biol Markers. 10.5301/ijbm.5000286.
  2. Huang L, et al. 2017. Oncotarget. 8(38): 64358.
  3. Isgro MA, et al. 2015. Adv Exp Med Biol. 867:125.
  4. Zaheer S, et al. 2013. Ann Indian Acad Neurol. 16(4):504.
Gene ID
2026 View all products for this Gene ID
UniProt
View information about NSE on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/26/2019

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