HRP anti-NrCAM Antibody

Pricing & Availability
Clone
N343/26 (See other available formats)
Other Names
Neuronal cell adhesion molecule, mBravo, nr-CAM, ng-CAM-related, neuronal surface protein Bravo, ngCAM-related cell adhesion molecule
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
N343slash26_HRP_NrCAM_Antibody_1_100918
Western blot of HRP anti-NrCAM antibody (clone N343/26). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebral cortex lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 5 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N343slash26_HRP_NrCAM_Antibody_1_100918
    Western blot of HRP anti-NrCAM antibody (clone N343/26). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebral cortex lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 5 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N343slash26_HRP_NrCAM_Antibody_2_100918
    IHC staining of HRP anti-NrCAM antibody (clone N343/26) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N343slash26_HRP_NrCAM_Antibody_3_100918
    IHC staining of HRP anti-NrCAM antibody (clone N343/26) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N343slash26_HRP_NrCAM_Antibody_4_100918
    IHC staining of HRP anti-NrCAM antibody (clone N343/26) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
821603 25 µg £67
Check Availability


Need larger quantities of this item?
Request Bulk Quote
821604 100 µg £114
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

NgCAM (Neuron-glia cell adhesion molecule)-related cell adhesion molecule (NrCAM), also known as Bravo, is an ankyrin-binding protein that modulates neuronal adhesion. NrCAM is part of the Ig superfamily and is involved in axonal cone growth.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 30-845 (extracellular domain) of mouse NrCAM.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783397 (BioLegend Cat. No. 821603)
AB_2783398 (BioLegend Cat. No. 821604)

Antigen Details

Structure
Expected MW: 125 kD
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Gene ID
319504 View all products for this Gene ID
UniProt
View information about NrCAM on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/11/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account