HRP anti-NMDAR2B Antibody

Pricing & Availability
Clone
N59/36 (See other available formats)
Other Names
GluN2B/NR2B glutamate receptor, Glutamate receptor ionotropic, NMDA 2B, NR2B, NMDAR2B, glutamate receptor, ionotropic, NMDA2B, N-methyl D-aspartate receptor subtype 2B, glutamate [NMDA] receptor subunit epsilon-2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
N59slash36_HRP_NMDAR2B_Antibody_1_070819
IHC staining of HRP anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2.5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N59slash36_HRP_NMDAR2B_Antibody_1_070819
    IHC staining of HRP anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2.5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N59slash36_HRP_NMDAR2B_Antibody_2_070819
    IHC staining of HRP anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N59slash36_HRP_NMDAR2B_Antibody_3_070819
    IHC staining of HRP anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N59slash36_HRP_NMDAR2B_Antibody_4_070819
    IHC staining of HRP anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
818703 25 µg £67
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818704 100 µg £114
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Description

Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 20-271 (extracellular, N-terminus) of rat GluN2B/NR2B glutamate receptor protein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is less reactive in human tissue in Western Blot

Antigen Details

Structure
Expected MW: 166 kD
Cell Type
Glutamatergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
24410 View all products for this Gene ID
UniProt
View information about NMDAR2B on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/08/2019

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