HRP anti-Neurofilament L (NF-L) Antibody

Pricing & Availability
Clone
NFL3 (See other available formats)
Other Names
Neurofilament Light Polypeptide 68kD, Neurofilament Light Polypeptide, Neurofilament Subunit NF-L, CMT1F, NFL, NEFL, NF-L
Isotype
Mouse IgG1, κ
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Product Citations
publications
NFL3_HRP_Neurofilament-L_Antibody_1_100218
IHC staining of HRP anti-Neurofilament L (NF-L) antibody (clone NFL3) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 40 µm
  • NFL3_HRP_Neurofilament-L_Antibody_1_100218
    IHC staining of HRP anti-Neurofilament L (NF-L) antibody (clone NFL3) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 40 µm
  • NFL3_HRP_Neurofilament-L_Antibody_2_100218
    IHC staining of HRP anti-Neurofilament L (NF-L) antibody (clone NFL3) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 40 µm
  • NFL3_HRP_Neurofilament-L_Antibody_3_100218
    IHC staining of HRP anti-Neurofilament L (NF-L) antibody (clone NFL3) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 40 µm
Cat # Size Price Quantity Avail. Save
845909 25 µg £64
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845910 100 µg £171
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Description

Neurofilaments (NF) are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Detection of NF, and the subunits of NF, may serve as a biomarker for axonal degeneration. The degree of axonal degeneration is related to the amount of NF detected in the cerebrospinal fluid (CSF) and the blood. Neurofilament light (NF-L) levels are elevated in Alzheimer’s Disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified macaque NFL.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783412 (BioLegend Cat. No. 845909)
AB_2783413 (BioLegend Cat. No. 845910)

Antigen Details

Structure
Neurofilament light is a 68 kD protein.
Distribution

Cytoskeleton and the cytoplasm.

Function
Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Zetterberg H, Blennow K. 2015. JAMA Neurol. 11:1237-8.
2. Bäckström D, et al. 2015. JAMA Neurol. 72(10):1175.
3. Janelidze S, et al. 2015. Neurology 85:1834.
4. Magdalinou NK, et al. 2015. J. Neurol. Neurosurg. Psychiatry 86(11):1240.
5. Skillbäck T, et al. 2014. Neurology. 83:1945.

Gene ID
4747 View all products for this Gene ID
UniProt
View information about Neurofilament L (NF-L) on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/03/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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