HRP anti-Myelin Protein Zero Antibody

Pricing & Availability
Clone
58A (See other available formats)
Other Names
MPZ, Myelin Protein Zero
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
58A_HRP_Myelin_Protein_Zero_Antibody_1_062119
Western blot of HRP anti-Myelin Protein Zero (clone 58A). Lane 1: Molecular weight marker; Lane 2: 5 µg of mouse sciatic nerve lysate; Lane 3: 10 µg of rat sciatic nerve lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 58A_HRP_Myelin_Protein_Zero_Antibody_1_062119
    Western blot of HRP anti-Myelin Protein Zero (clone 58A). Lane 1: Molecular weight marker; Lane 2: 5 µg of mouse sciatic nerve lysate; Lane 3: 10 µg of rat sciatic nerve lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 58A_HRP_Myelin_Protein_Zero_Antibody_2_062119
    IHC staining of HRP anti-Myelin Protein Zero (clone 58A) on formalin-fixed paraffin-embedded rat sciatic nerve tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 58A_HRP_Myelin_Protein_Zero_Antibody_3_062119
    IHC staining of HRP anti-Myelin Protein Zero (clone 58A) on formalin-fixed paraffin-embedded mouse sciatic nerve tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 58A_HRP_Myelin_Protein_Zero_Antibody_4_062119
    IHC staining of HRP anti-Myelin Protein Zero (clone 58A) on formalin-fixed paraffin-embedded human dorsal root ganglia tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
862203 25 µg £81
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862204 100 µg £178
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Description

Myelin protein zero (MPZ), is a membrane glycoprotein mainly expressed in the peripheral nervous system (PNS). It is the major structural component of the myelin sheath, and accounts for at least 50% of all proteins in the PNS. MPZ is expressed by Schewann cells. Mutations in MPZ have been associated with myelin deficiency, which may lead to neuropathies including charcot-Marie-Tooth and Dejerine-Sottas diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshimura K, et al. 1996. Neurosci Res. 25(1):41 (WB, IHC, ELISA)

Antigen Details

Structure
MPZ is a 248 amino acid protein with an apparent molecular mass of ~ 28 kD.
Distribution

Tissue Distribution: Peripheral nervous system
Cellular Distribution: Plasma membrane, extracellular, endoplasmic reticulum, and lysosome

Function
MPZ is an adhesion molecule involved in the formation of myelin sheath in the peripheral nervous system.
Interaction
Forms homodimer and homotetramer
Biology Area
Neurodegeneration, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules
Antigen References
  1. Chiò A and Lauria G. 2016. Lancet Neurol. 15(1):12
  2. Brennan KM, et al. 2015. Neurosci Lett. 596:14
  3. Tazir M, et al. 2014. J Neurol Sci. 347(1-2):14
Gene ID
4359 View all products for this Gene ID 17528 View all products for this Gene ID 24564 View all products for this Gene ID
UniProt
View information about Myelin Protein Zero on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/21/2019

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