HRP anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
SMI 99 (See other available formats)
Other Names
MBP, Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
SMI-99_HRP_MBP_Antibody_1_062119
Western blot of HRP anti-Myelin Basic Protein (clone SMI 99). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 μg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • SMI-99_HRP_MBP_Antibody_1_062119
    Western blot of HRP anti-Myelin Basic Protein (clone SMI 99). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 μg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • SMI-99_HRP_MBP_Antibody_2_062119
    IHC staining of HRP anti-Myelin Basic Protein (clone SMI 99) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
808405 25 µg £84
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808406 100 µg £178
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the CNS. MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of the classic human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. This antibody detects developing and adult myelin, developing oligodedrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections. The combination of this antibody with clone SMI-91 and/or SMI-94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Application References

(PubMed link indicates BioLegend citation)
  1. Fyffe-Maricich SL, et al. 2011. J. Neurosci. 31(3):843-50. (IHC, WB) PubMed
  2. Mendz GL, et al. 1985. Biochem. 228(1):61-8.
  3. Kerlero De Rosbo N, et al. 1984. Neurochem. Res. 9(10):1359-69.
  4. Carnegie PR, et al. 1983. J. Neuroimmunol. 5(2):125-34.
  5. Dowse CA, et al. 1983. J. Neuroimmunol. 5(2):135-44.
  6. Del Valle L,et al. Cancer Biol Ther. 9:286. (IHC) PubMed
  7. Kiryu-Seo S, et al. 2010. J. Neurosci. 30(19):6658-66. (ICC) PubMed
  8. Samanta J, et al. 2010. Stroke. 41(2):357-62. (IHC) PubMed
  9. Werner HB, et al. 2007. J. Neurosci. 27(29):7717-30. (WB, ICC) PubMed

Antigen Details

Cell Type
Oligodendrocytes
Biology Area
Cell Biology, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules
Gene ID
4155 View all products for this Gene ID 17196 View all products for this Gene ID 24547 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/21/2019

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