HRP anti-MFF Antibody

Pricing & Availability
Clone
N382/14 (See other available formats)
Other Names
Mitochondrial Fission Factor, C2orf33, Chromosome 2 Open Reading Frame 33, EMPF2, GL004
Isotype
Mouse IgG1, κ
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Product Citations
publications
N382-14_HRP_MMF_Antibody_1_081519
Western blot of HRP anti-MFF antibody (clone N382/14). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • N382-14_HRP_MMF_Antibody_1_081519
    Western blot of HRP anti-MFF antibody (clone N382/14). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
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857503 25 µg £67
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857504 100 µg £114
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Description

Mitochondrial fission factor (MFF) is a mitochondrion outer membrane protein that is part of the mitochondrial fission complex where it acts a receptor for the dynamin-related protein 1 (Drp1). The MFF-Drp1 complex is responsible for the promotion of mitochondrial fission. MFF has also been shown to regulate peroxisome morphology.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein including amino acids 1-173 and 272-322 of human MFF
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814578 (BioLegend Cat. No. 857503)
AB_2814579 (BioLegend Cat. No. 857504)

Antigen Details

Structure
MFF is a 342 amino acid protein with a molecular mass of ~38 kD.
Distribution

Tissue Distribution: Highly expressed in heart, kidney, liver, brain, muscle, and stomach.
Cellular Distribution: Mitochondria, peroxisome, nucleus, and cytosol.

Function
MFF plays a role in mitochondrial and peroxisomal fission by facilitating the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface.
Interaction
MFF interacts with the dynamin-related protein 1 (Drp1). MFF has also been shown to interact with DDX28 and ILF3.
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience
Molecular Family
Organelle Markers
Antigen References
  1. Moon Yong Cha, et al. 2018. PLoS Curr. 26;10.
  2. Kornfeld OS, et al. 2018. Sci Rep. 19;8(1):14034.
  3. Otera H, et al. 2010. J Cell Biol. 13;191(6):1141-58.
  4. Liu R, et al. 2015. Mol Biol Cell. 1;26(24):4466-77.
Gene ID
56947 View all products for this Gene ID
UniProt
View information about MMF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/15/2019

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