HRP anti-MAGUK (pan reactive) Antibody

Pricing & Availability
Clone
K28/86 (See other available formats)
Other Names
Disks large homolog 4, discs large homolog 4, Tax interaction protein 15, synapse-associated protein 90, postsynaptic density protein 95, post-synaptic density protein 95
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
K28-86_HRP_MAGUK_Antibody_1_100918
Western blot of HRP anti-MAGUK (pan-reactive) antibody (clone K28/86). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • K28-86_HRP_MAGUK_Antibody_1_100918
    Western blot of HRP anti-MAGUK (pan-reactive) antibody (clone K28/86). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • K28-86_HRP_MAGUK_Antibody_2_100918
    IHC staining of HRP anti-MAGUK (pan-reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • K28-86_HRP_MAGUK_Antibody_3_100918
    IHC staining of HRP anti-MAGUK (pan-reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
817803 25 µg £67
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817804 100 µg £114
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Description

Membrane-associated guanylate kinases (MAGUK) are a superfamily of scaffolding proteins defined by the presence of a guanylate kinase (GUK) domain. MAGUKs, such as PSD-95, SAP97, PSD-83, CASK, and MAGIs are involved in the synaptic development and function where they are important for the spatial organization of both pre- and postsynaptic proteins, including glutamate and other receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 77-299 of human PSD-95.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in Immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

This antibody is less reactive in human tissue in IHC-P

RRID
AB_2783388 (BioLegend Cat. No. 817803)
AB_2783389 (BioLegend Cat. No. 817804)

Antigen Details

Structure
Expected MW: 95-110 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
1742 View all products for this Gene ID
UniProt
View information about MAGUK (pan reactive) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/10/2018

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