HRP anti-HDAC2 Antibody

Pricing & Availability
Clone
3F3/HDAC2 (See other available formats)
Other Names
Histone deacetylase 2, YY1-associated factor 1, transcriptional regulator homolog RPD3, YAF1
Isotype
Mouse IgG1
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Product Citations
publications
3F3-HDAC2_HRP_HDAC2_Antibody_1_062119.png
Western blot of HRP anti-HDAC2 antibody (clone 3F3/HDAC2 ). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • 3F3-HDAC2_HRP_HDAC2_Antibody_1_062119.png
    Western blot of HRP anti-HDAC2 antibody (clone 3F3/HDAC2 ). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • 3F3-HDAC2_HRP_HDAC2_Antibody_2_062119.png
    Western blot of HRP anti-HDAC2 antibody (clone 3F3/HDAC2 ). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat liver lysate. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
815205 25 µg £81
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815206 100 µg £178
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Description

HDAC2 is a member of class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups (deacetylation) from the side chain of lysine residues on the N-terminal region of core histones (H2A, H2B, H3 and H4). Generally, histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.

HDAC2 lacks DNA binding activity and executes its function by recruiting transcription factors and forming large transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. HDAC2 also interacts in the late S-phase of DNA-replication with DNMT1 in transcriptional repressor complex composed of DNMT1, DMAP1, PCNA and CAF1.

In addition to histones, HDAC2 can deacetylate transcription factors and modify their transcriptional activity. HDAC2 has been shown to negatively regulate synaptic plasticity and memory formation. Elevated activity of HDAC2 has been known to associate with the loss of memory and neuronal degeneration. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminus of the human HDAC2 protein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only validated by western blot with the purified format.

Antigen Details

Structure
488 amino acids with a predicted molecular weight of 55.4 kD. It contains a histone deacetylase domain.
Distribution

Widely expressed in many cell types; nucleus.

Function
HDAC2 is a histone deacetylase responsible for the deacetylation of ε-amino acid group of lysine residues on the N-terminal tail of core histones (H2A, H2B, H3, H4). HDAC2 forms complex with transcription factors and acts as a transcriptional co-repressor.
Interaction
Forms histone deacetylase complexes with HDAC1, RBBP4, and RBBP7. Interacts with GFI1, SNW1, HDAC7, PRDM6, SAP30, SETDB1, SUV39H1, H2AFY, ATR, CBFA2T3, BCL-6, DNMT1, MINT, HDAC10, HCFC1, NRIP1, KDM4A, TSHZ3 and PELP1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Winter M, et al. 2013. EMBO J. 32:3176.
2. Ma P, et al. 2013. PLoS Genet. 9:e1003377.
3. Kurita M, et al. 2012. Nat. Neurosci. 15:1245.
4. Segre CV, et al. 2011. J. Biomed. Biotechnol. 2011:690848.
5. Brunmeir R, et al. 2009. Int. J. Dev. Biol. 53:275.
6. Guan JS, et al. 2009. Nature 459:55.

Gene ID
3066 View all products for this Gene ID
UniProt
View information about HDAC2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/21/2019

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