HRP anti-GABRB3 Antibody

Pricing & Availability
Clone
N87/25 (See other available formats)
Other Names
Gamma-aminobutyric acid receptor subunit beta-3, cleft palate 1, GABA(A) receptor subunit beta-3, gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
N87-25_HRP_GABRB3_Antibody_1_120418
Western blot of HRP anti-GABRB3 antibody (clone N87/25). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain membrane lysate; Lane 3: 20 μg of mouse brain membrane lysate; Lane 4: 20 μg of rat brain membrane lysate. The blot was incubated with 1 μg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N87-25_HRP_GABRB3_Antibody_1_120418
    Western blot of HRP anti-GABRB3 antibody (clone N87/25). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain membrane lysate; Lane 3: 20 μg of mouse brain membrane lysate; Lane 4: 20 μg of rat brain membrane lysate. The blot was incubated with 1 μg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N87-25_HRP_GABRB3_Antibody_2_120418
    IHC staining of HRP anti-GABRB3 antibody (clone N87/25) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 420902), the tissue was incubated with 1.0 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N87-25_HRP_GABRB3_Antibody_3_120418
    IHC staining of HRP anti-GABRB3 antibody (clone N87/25) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 420902), the tissue was incubated with 1.0 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
818503 25 µg £67
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818504 100 µg £114
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Description

Gamma-aminobutyric acid receptor subunit beta-3 (GABRB3) is a component of the hetero-pentameric receptor for gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the vertebrate brain. The receptor is a ligand-gated multi-subunit chloride channel. The GABRB3 protein is encoded by the GABRB3 gene in humans. Mutations in this gene have been associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, epilepsy and autism.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 370-433 of mouse GABA(A) Receptor, Beta-3 protein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783392 (BioLegend Cat. No. 818503)
AB_2783393 (BioLegend Cat. No. 818504)

Antigen Details

Structure
Expected Molecular Weight: 55 kD.
Cell Type
GABAergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
14402 View all products for this Gene ID
UniProt
View information about GABA(A)R, beta3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2018

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