HRP anti-FIG4/SAC3 Antibody

Pricing & Availability
Clone
N202/7 (See other available formats)
Other Names
SAC3, SAC domain-containing protein 3, Polyphosphoinositide phosphatase, Phosphatidylinositol 3,5-bisphosphate 5-phosphatase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N202-7_HRP_FIG4_SAC3_Antibody_082019
Western blot of HRP anti-anti-FIG4/SAC3 antibody (clone N202/7). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain membrane lysate; Lane 3: 20 μg of mouse brain membrane lysate; Lane 4: 20 μg of rat brain membrane lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • N202-7_HRP_FIG4_SAC3_Antibody_082019
    Western blot of HRP anti-anti-FIG4/SAC3 antibody (clone N202/7). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain membrane lysate; Lane 3: 20 μg of mouse brain membrane lysate; Lane 4: 20 μg of rat brain membrane lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
861803 25 µg £67
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861804 100 µg £114
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Description

Phosphoinositides (PIs) are signaling molecules that regulate vesicle trafficking in eukaryotic cells and are important for maintaining the nervous system. The levels of PIs are regulated by specific protein kinases and phosphatases. FIG4 protein (the homolog of yeast SAC3 protein), is a phosphatase well conserved from mammals to yeast, and is known to dephosphorylate phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) at the 5th position of inositol. By interacting with FAB1 and VAC14, the protein complex regulates the overall level of PI(3,5)P2. Loss of function of FIG4 leads to the reduction of the cellular PI(3,5)P2 levels. Mutations of human FIG4 gene cause autosomal recessive Charcot-Marie-Tooth type 4BJ (CMT4BJ) neuropathy with hereditary motor and sensory degeneration. Variants of FIG4 gene have also been found in patients with amyotrophic lateral sclerosis (ALS) and primary lateral sclerosis (PLS), suggesting it may be a risk factor for these disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein amino corresponding to amino acids 688-907 (C-terminus) of mouse FIG4.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814587 (BioLegend Cat. No. 861803)
AB_2814588 (BioLegend Cat. No. 861804)

Antigen Details

Structure
Human FIG4 is a 907 amino acid protein with a molecular mass of 103 kD.
Distribution

Tissue distribution: ubiquitously expressed.
Cellular source: plasma membrane, endoplasmic reticulim, endosome, golgi apparatus, intracellular membrane, and vacuoles.

Function
FIG4 is involved in the regulation of phosphoinositides levels and intracellular vesicle trafficking.
Interaction
Interacts with FAB1 and VAC14
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Protein Kinases/Phosphatase, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Chow C. et al. 2007. Nature. 448(7149):68-72.
  2. Chow C. et al. 2009. Am J Hum Genet. 84(1):85-8.
  3. Lenk G.M. et al. 2011. PLoS Genet. 7(6):e1002104.
Gene ID
9896 View all products for this Gene ID 103199 View all products for this Gene ID 309855 View all products for this Gene ID
UniProt
View information about FIG4/SAC3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/20/2019

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