HRP anti-FBXO7 Antibody

Pricing & Availability
Clone
W16207A (See other available formats)
Other Names
F-box only protein 7
Isotype
Rat IgG2a, κ
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W16207A_HRP_FBXO7_Antibody_1_100218
Western blot of HRP anti-FBXO7 antibody (clone A16207A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 0.055 µg/ml of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • W16207A_HRP_FBXO7_Antibody_1_100218
    Western blot of HRP anti-FBXO7 antibody (clone A16207A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 0.055 µg/ml of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • W16207A_HRP_FBXO7_Antibody_2_100218
    Western blot of HRP anti-FBXO7 antibody (clone A16207A). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate; Lane 3: 20 µg of Hela cell lysate. The blot was incubated with 0.055 µg/ml of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
853503 25 µg £84
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853504 100 µg £185
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Description

F-box only protein 7 (FBXO7) is involved in the ubiquitination process during protein degradation. FBXO7 is a subunit of the SCF (SKP1/cullin‐1/F‐box protein) complex. The scaffold proteins cullin‐1 and SKP1, the RING subunit Rbx1 together with the substrate‐recruiting subunit FBXO7 form a functional E3 ubiquitin ligase. FBXO7 is implicated in Parkinson’s disease. In addition to reduced proteasome activity, loss of FBXO7 in mice leads to Parkinsonism-like phenotype. Human FBXO7 is mainly localized to the nucleus, while the mutation observed in patients with parkinsonian-pyramidal syndrome leads to impaired nuclear localization of this protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human FBXO7 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for human FBXO7 and does not cross-react with rat or mouse FBXO7, validated by western blot. The two bands observed in western blot potentially indicate the two isoforms of FBXO7.

RRID
AB_2783431 (BioLegend Cat. No. 853503)
AB_2783432 (BioLegend Cat. No. 853504)

Antigen Details

Structure
FBXO7 is a 522 amino acid protein with a molecular mass of ~60 kD.
Distribution

Tissue distribution: FBXO7 is ubiquitously expressed in the many tissues including brain and muscle.
Cellular distribution: Cytoplasmic and nucleus.

Function
FBXO7 is involved in the ubiquitin-proteasome system, and defects in FBXO7 cause Parkinson's disease-like phenotypes.
Interaction
FBXO7 interacts with the members of the SCF (SKP1/cullin‐1/F‐box protein) complex.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Nuclear Markers
Antigen References
  1. Vingill S, et al. 2016. EMBO J. 35: 2008-25.
  2. Zhao T, et al. 2011. PLoS One. 6:e16983.
  3. Zhou ZD, et al. 2015. Hum. Mol. Genet. 24:6314-30.
Gene ID
25793 View all products for this Gene ID
UniProt
View information about FBXO7 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/03/2018

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