HRP anti-Ezrin Antibody

Pricing & Availability
Clone
CPTC-Ezrin-1 (See other available formats)
Other Names
Cytovillin, Cytovillin 2, CVIL, CVL, Villin-2, VIL2, p81, HEL-S-105
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
CPTC-Ezrin-1_HRP_Ezrin_Antibody_1_082019
Western blot of HRP anti-Ezrin antibody (clone CPTC-Ezrin-1). Lane 1: Molecular weight marker; Lane 2: 10 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 0.5 μg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • CPTC-Ezrin-1_HRP_Ezrin_Antibody_1_082019
    Western blot of HRP anti-Ezrin antibody (clone CPTC-Ezrin-1). Lane 1: Molecular weight marker; Lane 2: 10 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 0.5 μg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • CPTC-Ezrin-1_HRP_Ezrin_Antibody_2_082019
    IHC staining of HRP anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-Ezrin-1_HRP_Ezrin_Antibody_3_082019
    IHC staining of HRP anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-Ezrin-1_HRP_Ezrin_Antibody_4_082019
    IHC staining of HRP anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-Ezrin-1_HRP_Ezrin_Antibody_5_082019
    IHC staining of HRP anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-Ezrin-1_HRP_Ezrin_Antibody_6_082019
    IHC staining of HRP anti-Ezrin antibody (clone CPTC-Ezrin-1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
866403 25 µg £81
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866404 100 µg £178
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Description

Ezrin, a member of the ERM (Ezrin-Radixin-Moesin) family, is a linker protein between cytoskeleton and cellular membrane. It has wide temporal and spatial expression, and participates in a variety of cellular processes such as cell adhesion, cell motility, cell-cell communication, and apoptosis. High levels of Ezrin expression promotes tumorigenesis in tissues, including glioblastoma, breast cancer, and colon cancer. Ezrin is also highly expressed in neural stem cells and neuroblasts, where it may function in actin remodeling.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human Ezrin protein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814608 (BioLegend Cat. No. 866403)
AB_2814609 (BioLegend Cat. No. 866404)

Antigen Details

Structure
Human Ezrin is a 586 amino acid protein with a molecular mass of 70 kD.
Distribution

Tissue distribution: Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve
Cellular distribution: Cytoskeleton and plasma membrane

Function
Ezrin is involved in the connection of major cytoskeletal structures to the plasma membrane.
Interaction
Interacts with many proteins, such as MPP5, SLC9A3R2, MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B.
Cell Type
Astrocytes, Leukocytes, Neural Stem Cells, Neurons, Oligodendrocytes, T cells
Biology Area
Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Cytoskeletal Proteins, Scaffold Proteins
Antigen References
  1. Moon Y, et al. 2013. Histochem. Cell. Biol. 139:403-13.
  2. Xie JJ, et al. 2011. J. Histochem. Cytochem. 59(11):1001-8.
Gene ID
7430 View all products for this Gene ID
UniProt
View information about Ezrin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/20/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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