- P2C4 (See other available formats)
- Regulatory Status
- Other Names
- GJA1, Cx43, Gap Junction Protein Alpha 1
- Mouse IgG2a, κ
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- Product Citations
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Connexins are a family of transmembrane proteins that assemble to form vertebrate gap junctions, thus also called Gap Junction Proteins. So far, 21 Connexins have been discovered in humans. The gene GJA1 encodes Connexin 43 (Cx43), which is the most expressed Connexin, and can be found in many human tissues including skin, bone, and brain. Cx43 has been reported to be involved in synchronized contraction of the heart as well as embryonic development. Besides its functions as a channel protein, Cx43 also performs channel-independent functions. During brain and heart development, cytoplasmic Cx43 regulates the microtubule network and thus affects cell migration and polarity. In addition, mitochondrial GJA1 has also been reported to promote cell survival by down-regulating the intrinsic apoptotic pathway during conditions such as oxidative stress.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- KLH-conjugated peptide corresponding to amino acids 360-382 of Connexin 43.
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
WB - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunohistochemistry on paraffin-embedded tissue, a concentration range of 0.1 - 0.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody binds to the C-terminus of Connexin 43 and shows preference for non-phosphorylated Connexin 43 by western blotting.
(PubMed link indicates BioLegend citation)
- Chu G, et al. 2002. Cardiovasc. Res. 54(1):105. (WB)
- Wei CJ, et al. 2005. J. Biol. Chem. 280(20):19925. (WB)
AB_2728573 (BioLegend Cat. No. 849603)
AB_2728574 (BioLegend Cat. No. 849604)
- Connexin 43 (GJA1) is a 382 amino acid protein with a molecular mass of 43 kD.
Tissue distribution: Expressed in most tissues, enriched in brain, endocrine, muscle, bone marrow, immune System, ovaries, testes, and Skin.
Cellular distribution: Plasma membrane, cytosol, mitochondria, golgi apparatus, cytoskeleton and endoplasmic reticulum membrane.
- Connexin 43 plays a role in cell-to-cell communication by forming channels, or gap junctions between cells. It allows for the transport of nutrients, charged particles (ions), and other small molecules that carry necessary communication signals between cells.
- Molecular Family
- Gap Junction Proteins
- Antigen References
1. Grek CL, et al. 2016. Cancer Lett. 374(1):117.
2. Lilly E, et al. 2016. Semin. Cell Dev. Biol. Feb; 50:4
3. Michela P, et al. 2015. Eur. J. Pharmacol. 768:71-6.
- Gene ID
- 2697 View all products for this Gene ID
- View information about Connexin 43 360-382 on UniProt.org
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