- Clone
- ABL-93 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lysosome-associated membrane glycoprotein 2, lysosomal membrane glycoprotein 2, CD107 antigen-like family member B, lysosome-associated membrane protein 2
- Isotype
- Rat IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of HRP anti-CD107b (LAMP-2) antibody (clone ABL-93). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 10 µg of mouse kidney lysate .The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system. -
IHC staining of HRP anti-CD107b (LAMP-2) antibody (clone ABL-93) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 2.5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Staining of the choroid plexus region is shown. Scale bar: 50 µm -
IHC staining of HRP anti-CD107b (LAMP-2) antibody (clone ABL-93) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 816103 | 25 µg | £82.00 | ||||
| 816104 | 100 µg | £181.00 | ||||
CD107b, also known as LAMP-2, is a 105 kD, highly glycosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, on the surface of activated platelets, activated lymphocytes and some tumor cell lines. LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- This monoclonal antibody was raised against Balb/c 3T3 mouse embryo fobroblast tissue culture cell glycoproteins. It has been mapped to the N-terminus of LAMP 2.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).
This antibody is specific to mouse LAMP-2. LAMP-2 molecular weight appears to be at ~105 kDa on the gel due to high glycosylation. - Product Citations
- RRID
-
AB_2783386 (BioLegend Cat. No. 816103)
AB_2783387 (BioLegend Cat. No. 816104)
Antigen Details
- Structure
- LAMP-2 is a 415 amino acid protein with a molecular mass of 45 kD.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- CD Molecules
- Gene ID
- 16784 View all products for this Gene ID
- UniProt
- View information about CD107b on UniProt.org
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