HRP anti-CD107b (LAMP-2) Antibody

Pricing & Availability
Clone
ABL-93 (See other available formats)
Other Names
Lysosome-associated membrane glycoprotein 2, lysosomal membrane glycoprotein 2, CD107 antigen-like family member B, lysosome-associated membrane protein 2
Isotype
Rat IgG2a
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Product Citations
publications
ABL-93_HRP_CD107b_LAMP2_1_111618
Western blot of HRP anti-CD107b (LAMP-2) antibody (clone ABL-93). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 10 µg of mouse kidney lysate .The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • ABL-93_HRP_CD107b_LAMP2_1_111618
    Western blot of HRP anti-CD107b (LAMP-2) antibody (clone ABL-93). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 10 µg of mouse kidney lysate .The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • ABL-93_HRP_CD107b_LAMP2_2_111618
    IHC staining of HRP anti-CD107b (LAMP-2) antibody (clone ABL-93) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 2.5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Staining of the choroid plexus region is shown. Scale bar: 50 µm
  • ABL-93_HRP_CD107b_LAMP2_3_111618
    IHC staining of HRP anti-CD107b (LAMP-2) antibody (clone ABL-93) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
816103 25 µg £81
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816104 100 µg £178
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Description

CD107b, also known as LAMP-2, is a 105 kD, highly glycosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, on the surface of activated platelets, activated lymphocytes and some tumor cell lines.  LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against Balb/c 3T3 mouse embryo fobroblast tissue culture cell glycoproteins. It has been mapped to the N-terminus of LAMP 2.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

This antibody is specific to mouse LAMP-2. LAMP-2 molecular weight appears to be at ~105 kDa on the gel due to high glycosylation.

RRID
AB_2783386 (BioLegend Cat. No. 816103)
AB_2783387 (BioLegend Cat. No. 816104)

Antigen Details

Structure
LAMP-2 is a 415 amino acid protein with a molecular mass of 45 kD.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
CD Molecules
Gene ID
16784 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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