HRP anti-Ankyrin-R Antibody

Pricing & Availability
Clone
N388A/10 (See other available formats)
Other Names
ANK1, Ankyrin-R
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
N388A-10_HRP_Ankyrin-R_Antibody_061819.png
Western blot of HRP anti-Ankyrin-R antibody (clone N388A/10). Lanes 1, 3: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 4: 20 µg of mouse brain lysate; Lane 5: 20 µg of rat brain lysate. The blots were incubated with 2 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N388A-10_HRP_Ankyrin-R_Antibody_061819.png
    Western blot of HRP anti-Ankyrin-R antibody (clone N388A/10). Lanes 1, 3: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 4: 20 µg of mouse brain lysate; Lane 5: 20 µg of rat brain lysate. The blots were incubated with 2 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Avail. Save
857703 25 µg £67
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857704 100 µg £114
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Description

Ankyrin-R is an anchor protein whose main role in vivo is to anchor integral membrane protein/complexes to the cytoskeleton. It was originally discovered in erythrocytes, but later to be found expressed in the brain. The gene encoding Ankyrin-R, ANK1, has a highly complex regulatory mechanism, which gives rise to a variety of different isoforms of Ankyrin-R. Recent studies in Alzheimer’s Disease have shown that epigenetic dysregulation of ANK1, in combination of several other proteins including Trem-2, could be a contributing factor of increased TNFA expression and neuroinflammation1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal condition
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Taylor A, et al. 2017. Front Cell Neurosci. 11:11 (IHC-F)
  2. Saifetiarova J, et al. 2017. J Neurosci. 37(10):2524 (IHC-F)
  3. Taylor A, et al. 2018. eNeuro. 5(3) (IHC-F, WB)

Antigen Details

Structure
ANK1 is a 1881 amino acid protein with an apparent molecular mass of ~ 206 kD.
Distribution

Tissue Distribution: Brain and bone marrow.
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, nucleus, and endoplasmic reticulum.

Function
ANK1 functions as an adaptor protein to anchor integral membrane proteins/complexes to the cytoskeleton.
Interaction
SPTB, SLC4A1, EPB41, TTN, OBSCN, HIF1AN
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adaptor Proteins
Antigen References
  1. Morris G, et al. 2018. Mol Neurobiol. doi: 10.1007/s12035-018-1092-y.
  2. Nicolas V, et al. 2006. Transfuse Clin Biol. 13(1-2):23
  3. Kline CF, et al. 2006. Expert Rev Cardiovasc Ther. 4(4):477
Gene ID
286 View all products for this Gene ID 11733 View all products for this Gene ID 306570 View all products for this Gene ID
UniProt
View information about Ankyrin-R on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/18/2019

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