HRP anti-Alpha-II Spectrin Antibody

Pricing & Availability
Clone
D8B7 (See other available formats)
Other Names
Spectrin alpha chain, brain, alpha-II spectrin, fodrin alpha chain, spectrin, non-erythroid alpha chain, EL2, SPTA1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
D8B7_HRP_AlphaIISpectrin_Antibody_1_WB_062419.png
Western blot of HRP anti-Alpha-II-Spectrin antibody (clone D8B7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system. The lower band in lane 2 is spectrin breakdown product (SBDP).
  • D8B7_HRP_AlphaIISpectrin_Antibody_1_WB_062419.png
    Western blot of HRP anti-Alpha-II-Spectrin antibody (clone D8B7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system. The lower band in lane 2 is spectrin breakdown product (SBDP).
  • D8B7_HRP_AlphaIISpectrin_Antibody_2_WB_062419.png
    IHC staining of HRP anti-Alpha-II-Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D8B7_HRP_AlphaIISpectrin_Antibody_3_WB_062419.png
    IHC staining of HRP anti-Alpha-II-Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D8B7_HRP_AlphaIISpectrin_Antibody_4_WB_062419.png
    IHC staining of HRP anti-Alpha-II-Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
803207 25 µg £84
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803208 100 µg £185
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Description

The spectrins are a family of widely distributed cytoskeletal proteins which are involved in actin crosslinking. Spectrin α-chain heterodimerizes with tissue-specific β-chains. It is specifically expressed in nonerythrocytic cells. The protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the gene are the cause of early infantile epileptic encephalopathy. Fodrin, a spectrin that seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)4, 6, 8. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.

This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.

Reactivity to Drosophila was only validated with the purified format.

Application References

(PubMed link indicates BioLegend citation)
  1. Konieczny P, et al. 2008. J Cell Biol. 181:667-81
  2. Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
  3. Xu J, et al. 2000. J Cell Science. 113:3805-14
  4. De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
  5. Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
  6. Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
  7. Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
  8. Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
  9. Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed

Antigen Details

Structure
Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Presynaptic proteins, Scaffold Proteins
Antigen References
  1. Pike BR, et al. 2001. J Neurochem. 78(6):1297-306.
  2. Weiss ES, et al. 2009. Ann Thorac Surg. 88(2):543
Gene ID
6709 View all products for this Gene ID
UniProt
View information about Alpha-II Spectrin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/24/2019

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