Go-ChIP-Grade™ Purified anti-STAT6 Phospho (Tyr641) Antibody

Pricing & Availability
Clone
A15137A (See other available formats)
Other Names
Signal transducer and activator of transcription 6, IL-4 Stat
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_A15137A_GoChIP_STAT6_Antibody_1_011518
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from Ramos cells starved overnight and then treated with IL-4 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT6 Phospho (Tyr641) (clone A15137A), or B) equal amount of Purified mouse Ig1, κ isotype control antibody (Cat. No. 401401), or C) competitor's ChIP-grade purified anti-STAT6 Phospho (Tyr641) antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human DMD gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 1_A15137A_GoChIP_STAT6_Antibody_1_011518
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from Ramos cells starved overnight and then treated with IL-4 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT6 Phospho (Tyr641) (clone A15137A), or B) equal amount of Purified mouse Ig1, κ isotype control antibody (Cat. No. 401401), or C) competitor's ChIP-grade purified anti-STAT6 Phospho (Tyr641) antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human DMD gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • A15137A_PURE_STAT6phospho_1_WB_Antibody_050318
    Total cell lysate (15 µg protein) from non-treated and 100 ng/mL recombinant human IL-4 treated Daudi cells (10 min treatment) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) and 1:50000 (0.01 µg/mL) diluted purified anti-STAT6 phospho (Tyr641) antibody (clone A15137A) (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • A15137A_PURE_STAT6phospho_2_IF_Antibody_081717
    Daudi cells un-treated and treated with 100 ng/mL recombinant human IL-4 (Cat. No. 574004) were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1 µg/ml anti-STAT6 phospho (Tyr641) antibody (clone A15137A) overnight at 4 degree followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • A15137A_PURE_STAT6phospho_3_IF_Antibody_081717
Cat # Size Price Quantity Avail. Save
690103 25 µg £58
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690104 100 µg £168
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Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT6 peptide phosphorylated at Tyr641.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunofluorescent staining protocol, the recommended usage is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms at 74, 82, and 94 kD. The predominant band detected is at 94 kD.

RRID
AB_2728511 (BioLegend Cat. No. 690103)
AB_2728512 (BioLegend Cat. No. 690104)

Antigen Details

Structure
847 amino acids, predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
Distribution

Translocates to the nucleus when phosphorylated.

Function
STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling.
Interaction
STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Nuclear Markers
Antigen References
  1. Goenka S, et al. 2011. Immunol. Res. 50:87
  2. Wurster AL, et al. 2000. Oncogene. 19:2577
  3. Akira S, 1999. Stem Cells. 17:138
  4. Zamorano J, et al. 2005. J. Immunol. 174:2843
  5. David M, et al. 2001. Oncogene. 20:6660
  6. Takeda K, et al. 1996. Nature. 380:627
Gene ID
6778 View all products for this Gene ID
UniProt
View information about STAT6 Phospho (Tyr641) on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/03/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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