Go-ChIP-Grade™ Purified anti-Histone H4 Monomethyl (Lys20) Antibody

Pricing & Availability
Clone
5E10-D8 (See other available formats)
Other Names
Histone H4, Histone 1, H4a, H4 histone family member A
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
1_5E10-D8_GoChIP_HistoneH4_Monomethyl_Antibody_121416
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Histone H4 Monomethyl (Lys20) (clone 5E10-D8), B) equal amount of Purified Mouse IgG1 Isotype Control Antibody (clone MG1-45), or C) competitor's ChIP-grade Purified anti-Histone H4 Monomethyl (Lys20) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 1_5E10-D8_GoChIP_HistoneH4_Monomethyl_Antibody_121416
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Histone H4 Monomethyl (Lys20) (clone 5E10-D8), B) equal amount of Purified Mouse IgG1 Isotype Control Antibody (clone MG1-45), or C) competitor's ChIP-grade Purified anti-Histone H4 Monomethyl (Lys20) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 2_5E10-D8_Purified_Histone-H4_Antibody_1_122118
    IHC staining of purified anti-Histone H4 Monomethyl (Lys20) antibody (clone 5E10-D8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_5E10-D8_Purified_Histone-H4_Antibody_2_122118
    IHC staining of purified anti-Histone H4 Monomethyl (Lys20) antibody (clone 5E10-D8) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_5E10-D8_Purified_Histone-H4_Antibody_3_122118
    IHC staining of purified anti-Histone H4 Monomethyl (Lys20) antibody (clone 5E10-D8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5_5E10-D8_PURE_Histone_H4_Antibody_WB_011618
    Total lysates (15µg protein) from Jurkat (lane1), Raw 264.7 (lane 2) and UMR106 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:5000 diluted (0.1 µg/mL) Purified anti-Histone H4 Monomethyl (Lys20) Antibody, clone 5E10-D8 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-Histone H4 Monomethyl (Lys20) Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
828003 25 µg £51
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828004 100 µg £161
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing methylated lysine 20 of human Histone H4.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IHC-P, WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution range of 1:100 - 1:200 is suggested. For Western blotting, the suggested use of this reagent is 0.1 µg/mL (1:5000).  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

25 µg and 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-5 or 10-20 immunoprecipitations, respectively, at the recommended dilutions.

Application References

(PubMed link indicates BioLegend citation)
  1. Shirakata Y, et al. 2014. J. Reprod. Dev. 60:383. (IHC-P)
RRID
AB_2632897 (BioLegend Cat. No. 828003)
AB_2632843 (BioLegend Cat. No. 828004)

Antigen Details

Structure
Histone H4 is a 103 amino acid protein with an apparent molecular mass of 12 kD
Distribution

Nucleus.

Function
Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Interaction
Various transcription factors and nuclear protein.
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Stem Cells, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology. 409:441.
2. Wang Z, et al. 2008. Nat. Genet. 40:897.
3. Beck DB, et al. 2012. Genes Dev. 26:325.

Gene ID
8359 View all products for this Gene ID
UniProt
View information about Histone H4 Monomethyl (Lys20) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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