- Clone
- Poly6089 (See other available formats)
- Other Names
- Histone H3 Di-/Trimethy (Lys 36)
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HeLa cells with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-Histone H3 Di-/Tri-methyl (Lys36) antibody, or B) equal amount of Go-ChIP-Grade™ purified rabbit polyclonal isotype control antibody, clone Poly29108 (Cat. No. 402211) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 910805). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin. -
Specificity of Go-ChIP-Grade™ purified anti-Histone H3 Di-/Tri-methyl (Lys36) antibody (clone Poly6089, Cat. No. 608903) was determined by peptide ELISA. A variety of histone modification peptides including H3K4me1, H3K4me2, H3K4me3, H3K36me1, H3K36me2 and H3K36me3 were pre-coated at 25 ng/mL and incubated with increasing concentrations of Go-ChIP-Grade™ purified anti-Histone H3 Di-/Tri-methyl (Lys36) antibody. Go-ChIP-Grade™ purified anti-Histone H3 Di-/Tri-methyl (Lys36) antibody binds to H3K36me2 and H3K36me3 peptide in a dose-dependent manner with an ED50 of 0.9 ng/mL and 0.18 ng/mL, respectively.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 608903 | 50 µl | £74 | ||||
| 608904 | 200 µl | £175 | ||||
Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.
Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modification plays critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing, chromosome condensation and some diseases including autoimmune diseases and cancers.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Synthetic peptide correspond¬ing to the N-terminus of histone H3 tri-methylated at Lysine 36.
- Formulation
- Phosphate-buffered solution/50% glycerol/0.09% NaN3.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored at -20°C.
- Application
-
ChIP - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:500 by volume. It is recommended that the reagent be titrated for optimal performance before each experiment.
- Application Notes
-
Clone Poly6089 is predicted to recognize both mouse and rat histone H3 tri-methylated at Lysine 36 due to complete sequence homology between the immunizing peptide and the mouse homolog. This clone displayed poor sensitivity when tested for western blot. We therefore do not recommend the clone for this application.
- RRID
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AB_2783357 (BioLegend Cat. No. 608903)
AB_2783358 (BioLegend Cat. No. 608904)
Antigen Details
- Structure
- Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
- Distribution
-
Nucleus
- Function
- Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
- Interaction
- H3K36me2/3 is found in actively transcribed gene bodies.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Antigen References
-
- Chen HM, et al. 2015. Epigenetics and Dermatology 409.
- Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone H3 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
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