GluA1/2 Receptor Antibody Sampler Kit

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1-K28slash74_PURE_PSD95_WB_021218.png
Western blot of purified anti-PSD95 antibody (clone K28/74). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 1-K28slash74_PURE_PSD95_WB_021218.png
    Western blot of purified anti-PSD95 antibody (clone K28/74). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 2-K28slash74_PURE_PSD95_Mu_IHCP_2_070218.png
    IHC staining of purified anti-PSD95 antibody (clone K28/74) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 555 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50
  • 3-L21slash32_Purified_GluR2_Antibody_3_IHC_112717.png
    IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm
  • 4-N355slash1_Purified_GluR1_Antibody_1_031618.png
    Western blot of purified anti-GluR1 antibody (clone N355/1). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5-L208_PURE_PICK1_Antibody_1_101918
    Western blot of purified anti-PICK1 antibody (clone L20/8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 6-L42slash17_PURE_SynDIG1_Antibody_032718.png
    Western blot of anti-SynDIG1 antibody (clone L42/17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human frontal cortex lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of L42/17 or mouse IgG2a, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
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899907 1 kit £188
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Description

The GluA1/2 Receptor Antibody Sampler Kit offers flexibility for sampling and detection of AMPA receptor subunits GluA1 and GluA2, postsynaptic marker PSD95, and proteins involved in the regulation of AMPA receptor content; PICK1 and SynDYG1. These antibodies are highly suitable for WB and IHC applications.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype
Anti-PSD95 K28/74 25 μl Human, Mouse, Rat Mouse IgG1
Anti-GluR2  L21/32 25 μl Human, Mouse, Rat Mouse IgG1
 Anti-GluR1  N355/1 25 μl Mouse, Rat Mouse IgG1
 Anti-PICK1 L20/8 25 μl Human, Mouse, Rat Mouse IgG2a
 Anti-SynDIG1 L42/17 25 μg Human, Mouse, Rat Mouse IgG2a, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Antibody Type
Monoclonal
Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB, IHC-P - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-PSD95: 0.1 - 5.0 µg/ml
Anti-GluR1: 2.0 - 10 µg/ml
Anti-PICK1: 1.0 - 5.0 µg/ml
Anti-SynDIG1: 1.0 - 5.0 µg/ml

For immunohistochemistry on formalin-fixed paraffin-embedded tissue, the suggested uses of these reagents are as follows:

Anti-PSD95: 2.0 - 10 µg/ml
Anti-GluR2: 1.0 - 10 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

Please refer to individual product data sheets for antigen references.

Gene ID
50592 View all products for this Gene ID 29627 View all products for this Gene ID 1742 View all products for this Gene ID 84591 View all products for this Gene ID 79953 View all products for this Gene ID

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Go To Top Version: 1    Revision Date: 10/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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