FITC anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
GL7_FITC_GL7_Antibody_FC_1_110512
Balb/c mouse bone marrow cells were stained with IgD Pacific Blue™ and GL7 (clone GL7, top) FITC or rat IgM, κ FITC isotype control (bottom).
  • GL7_FITC_GL7_Antibody_FC_1_110512
    Balb/c mouse bone marrow cells were stained with IgD Pacific Blue™ and GL7 (clone GL7, top) FITC or rat IgM, κ FITC isotype control (bottom).
  • GL7_FITC_GL7_Antibody_FC_2_110512
Compare all formats See FITC spectral data
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144603 50 µg £62
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144604 500 µg £233
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  2. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
  3. Pankhurst TE, et al. 2023. Cell Rep. 42:112310. PubMed
  4. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  5. Menolfi D, et al. 2023. Nat Commun. 14:3618. PubMed
  6. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  7. Hu Q, et al. 2022. Cell Rep. 40:111035. PubMed
  8. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  9. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  10. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  11. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  12. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  13. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  14. Tam H, et al. 2016. Proc Natl Acad Sci U S A. 113: E6639 - E6648. PubMed
  15. Yang D, et al. 2020. Nat Commun. 2.411111111. PubMed
  16. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  17. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  18. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  19. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  20. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  21. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  22. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  23. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  24. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  25. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  26. Beloor J et al. 2018. Cell host & microbe. 23(4):549-556 . PubMed
  27. Guo C, et al. 2021. Cell Rep Med. 2:100448. PubMed
  28. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  29. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  30. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  31. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  32. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  33. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  34. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  35. Yang C, et al. 2020. Cell Host Microbe. 467:27. PubMed
  36. Sisteré–Oró M, et al. 2020. Vet Res. 51:57:00. PubMed
  37. Voigt A, et al. 2016. Sci Rep. 6:38717. PubMed
  38. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  39. Cooley L, et al. 2016. Sci Rep. 6: 25840. PubMed
  40. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  41. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  42. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  43. Chen Z, et al. 2020. J Immunol. 204:335. PubMed
  44. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
  45. Nasrallah R, et al. 2020. Nature. 583:447. PubMed
  46. BJ L, et al. 2017. J Exp Med . 10.1084/jem.20161461. PubMed
RRID
AB_2561696 (BioLegend Cat. No. 144603)
AB_2561697 (BioLegend Cat. No. 144604)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
UniProt
View information about GL7 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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