FITC anti-human HLA-A,B,C Antibody

Pricing & Availability
W6/32 (See other available formats)
Regulatory Status
Other Names
Major Histocompatibility Class I, MHC class I
Mouse IgG2a, κ
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Product Citations
Human peripheral blood lymphocytes stained with W6/32 FITC
  • W632_FITC_072607
    Human peripheral blood lymphocytes stained with W6/32 FITC
Compare all formats See FITC spectral data
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311403 25 tests £57
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311404 100 tests £129
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MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.

Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Darrow TL, et al. 1989. J. Immunol. 142:3329.
  2. Stern P, et al. 1987. J. Immunol. 138:1088.
  3. Tran TM, et al. 2001. Immunogenetics 53:440.
  4. Barbatis C, et al. 1981. Gut 22:985.
  5. Ayyoub M, et al. 2004. Cancer Immunity 4:7.
  6. DeFelice M, et al. 1990. Cell. Immunol. 126:420.
  7. Fayen J, et al. 1998. Int. Immunol. 10:1347.
  8. Turco MC, et al. 1988. J. Immunol. 141:2275.
  9. Geppert TD, et al. 1989. J. Immunol. 142:3763.
  10. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  11. Nagano M, et al. 2007. Blood 110:151.
  12. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
  13. Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
  14. Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
  15. Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
  16. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  17. Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
  18. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
  19. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
  20. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
  21. Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations
  1. Oreskovic E, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  2. Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed
  3. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  4. Abreu SC, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 319:L908. PubMed
  5. Robinson A, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0540-3. PubMed
  6. Vishnoi M, et al. 2018. Cancer Res. 78:5349. PubMed
  7. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  8. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  9. McDaid WJ, et al. 2021. Nanoscale. 13:15010. PubMed
  10. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  11. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  12. Tallerico R, et al. 2017. Oncoimmunology. 6:e1284718. PubMed
  13. Sharma R, et al. 2021. Nat Commun. 12:472. PubMed
  14. Robinson A, et al. 2015. PLoS One. 10: 0139023. PubMed
  15. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  16. Yumoto K, et al. 2016. Sci Rep. 6:36520. PubMed
  17. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  18. Liu X, et al. 2017. International Immunopharmacology. 10.1016/j.intimp.2017.04.012. PubMed
  19. Wei C, et al. 2012. J Allergy Clin Immunol. 129:1562. PubMed
  20. Chen X, et al. 2015. Mol Immunol. 65:34. PubMed
  21. Meng J, et al. 2010. Clin Vaccine Immunol. 1.325694444. PubMed
  22. Yin Z, et al. 2012. J Immunol. 189:2735. PubMed
  23. Tangtanatakul P, et al. 2017. Sci Rep. 7:14517. PubMed
  24. Takahashi A, et al. 2021. Front Oncol. 11:707473. PubMed
  25. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  26. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
  27. Ng S, et al. 2022. Sci Rep. 12:14087. PubMed
  28. Tallerico R, et al. 2013. J Immunol. 190:2381. PubMed
  29. Chew GL et al. 2019. Dev Cell. 50(5):658-671 . PubMed
  30. Song L, et al. 2021. J Invest Dermatol. 141:903. PubMed
  31. Qi YA, et al. 2021. Mol Cell Proteomics. 20:100136. PubMed
  32. Mirlashari MR, et al. 2021. Proteomics Clin Appl. 15:e2100022. PubMed
  33. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
  34. Ritter C, et al. 2017. Scientific Reports. 10.1038/s41598-017-02608-0. PubMed
  35. Nosaka K, et al. 2021. Anticancer Res. 41:4741. PubMed
  36. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
AB_314872 (BioLegend Cat. No. 311403)
AB_314873 (BioLegend Cat. No. 311404)

Antigen Details

Ig superfamily

All nucleated cells

Antigen presentation
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
3105 View all products for this Gene ID
View information about HLA-A on

Related FAQs

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Go To Top Version: 2    Revision Date: 01/16/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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