FITC anti-human CD63 Antibody

Pricing & Availability
H5C6 (See other available formats)
Regulatory Status
HCDM listed
Other Names
LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • H5C6_FITC_082911
    Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
353005 25 tests £68
Check Availability

Need larger quantities of this item?
Request Bulk Quote
353006 100 tests £173
Check Availability

Need larger quantities of this item?
Request Bulk Quote

CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
T cell line HPB-ALL
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
  2. Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
Product Citations
  1. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
  2. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  3. Eldh M, et al. 2021. Cancers (Basel). 13:. PubMed
  4. Otahal A, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Dietze R, et al. 2021. Theranostics. 11:1377. PubMed
  6. Qi Z, et al. 2021. Front Immunol. 12:732209. PubMed
  7. Saha B, Pandey N, Battacharya S 2017. Journal of Proteomics. 10.1016/j.jprot.2017.05.021. PubMed
  8. Best MG et al. 2017. Cancer cell. 32(2):238-252 . PubMed
  9. Welch J, Madison M 2017. Sci Rep. 10.1038/srep45034. PubMed
  10. Sung PS, et al. 2019. Nat Commun. 10:2402. PubMed
  11. Otahal A, et al. 2020. Front Bioeng Biotechnol. 8:584050. PubMed
  12. Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed
  13. Yong T, et al. 2019. Nat Commun. 10:3838. PubMed
  14. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  15. An S, et al. 2013. PLoS One. 8:81377. PubMed
  16. Chan J, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31435-X. PubMed
  17. Slovick A, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31186-1. PubMed
  18. Marsden AJ, et al. 2021. ACS Pharmacol Transl Sci. 4:1567. PubMed
  19. Otahal A, et al. 2021. Sci Rep. 5823:11. PubMed
  20. de Pedro MÁ, et al. 2021. Int J Mol Sci. 22:. PubMed
AB_10933264 (BioLegend Cat. No. 353005)
AB_10898319 (BioLegend Cat. No. 353006)

Antigen Details

Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle

Platelet activation
CD9, CD81, VLA-3, and VLA-6
Cell Type
B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Azorsa DO, et al. 1991. Blood 78:280.
  2. Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
  3. Hildreth JE, et al. 1991. Blood 77:121.
  4. Anzai N, et al. 2002. Blood 99:4413.
Gene ID
967 View all products for this Gene ID
View information about CD63 on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/27/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD63

  • FITC anti-human CD63

  • Pacific Blue™ anti-human CD63

  • APC anti-human CD63

  • PE/Cyanine7 anti-human CD63

  • Alexa Fluor® 647 anti-human CD63

  • Biotin anti-human CD63

  • PerCP/Cyanine5.5 anti-human CD63

  • PE/Dazzle™ 594 anti-human CD63

  • Brilliant Violet 421™ anti-human CD63

  • Brilliant Violet 650™ anti-human CD63

  • APC/Fire™ 750 anti-human CD63

  • Alexa Fluor® 700 anti-human CD63

  • Brilliant Violet 510™ anti-human CD63

  • Alexa Fluor® 594 anti-human CD63

  • TotalSeq™-A0404 anti-human CD63

  • Alexa Fluor® 488 anti-human CD63

  • Ultra-LEAF™ Purified anti-human CD63

  • Brilliant Violet 711™ anti-human CD63

  • Brilliant Violet 785™ anti-human CD63

  • APC/Cyanine7 anti-human CD63

  • TotalSeq™-B0404 anti-human CD63

  • TotalSeq™-C0404 anti-human CD63

  • Brilliant Violet 605™ anti-human CD63 Antibody

  • PE/Cyanine5 anti-human CD63 Antibody


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account