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FITC anti-human CD177 Antibody

Pricing & Availability
Clone
MEM-166 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1
Isotype
Mouse IgG1, κ
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Product Citations
publications
MEM-166_FITC_020708
Human peripheral blood granulocytes stained with MEM-166 FITC
  • MEM-166_FITC_020708
    Human peripheral blood granulocytes stained with MEM-166 FITC
Compare all formats See FITC spectral data
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315803 25 tests £70
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315804 100 tests £157
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Description

CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human granulocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.

Application References

(PubMed link indicates BioLegend citation)
  1. Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press.
  2. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed
  3. Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed
  4. von Vietinghoff S, et al. 2007. Blood 109:4487. (IF)
  5. Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)
Product Citations
  1. Brachemi S, et al. 2007. J Am Soc Nephrol. 2.368055556. PubMed
  2. Vijayakumar B, et al. 2022. Immunity. . PubMed
  3. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  4. Esmaeili B, et al. 2022. J Immunol Res. 2022:4387555. PubMed
RRID
AB_2072604 (BioLegend Cat. No. 315803)
AB_2072603 (BioLegend Cat. No. 315804)

Antigen Details

Structure
uPAR family, GPI-linked cell surface glycoprotein, 60 kD
Distribution

Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)

Function
Antigen involved in neutrophil allo- and autoimmunity, function unknown
Modification
Glycosylated
Cell Type
Granulocytes, Hematopoietic stem and progenitors, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002)
2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301.
3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108.
4. Temerinac S, et al. 2000. Blood 95:2569.

Gene ID
57126 View all products for this Gene ID
UniProt
View information about CD177 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03/18/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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