Filaggrin Polyclonal Antibody, Purified

Pricing & Availability
Clone
Poly19058 (See other available formats)
Other Names
Epidermal filaggrin
Previously
Covance Catalog# PRB-417P
Isotype
Rabbit Polyclonal
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Product Citations
publications
Purified_Filaggrin_Antibody_IHC_021715
Staining of Filaggrin (clone Poly19058) on frozen normal human skin tissue.
  • Purified_Filaggrin_Antibody_IHC_021715
    Staining of Filaggrin (clone Poly19058) on frozen normal human skin tissue.
Cat # Size Price Quantity Avail. Save
905801 100 µl £251
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19058 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Filaggrin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Reactivity
Human, Mouse, Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide corresponding to residues 24-39 in the partial sequence of filaggrin.
Formulation
Phosphate-buffered solution+ 0.03% Thimerosal.
Preparation
The antibody was purified by peptide affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:500 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Frozen tissue sections
Pretreatment: Not Required
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC). This antibody has been used successfully on frozen sections.

Positive Control Tissue: Normal skin


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wallace L, Roberts-Thompson L, and Reichelt J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci., 125: 1750-1758, Apr 2012. PubMed
  2. Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and PTrez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. [IHC] PubMed
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
  6. Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
  7. DiTommaso T, et al. 2014. PLoS Genet. 10:1004706. (IHC) PubMed
  8. Liakath-Ali K, et al. 2014. Nat Commun. 11:3540. (IHC) PubMed
Product Citations
  1. Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
  2. Wallace L, et al. 2012. J Cell Sci. 125:1750-1758. PubMed
  3. Bayo P, et al. 2008. Endocrinology. 149:1377-1388. PubMed
  4. DiTommaso T, et al. 2014. PLoS Genet. 10:e1004706. PubMed
  5. Liakath-Ali K, et al. 2014. Nat Commun. 5:3540. PubMed
  6. Hobbs R, et al. 2016. Oncogene. 10.1038/onc.2016.102. PubMed
  7. Muroyama A, Seldin L, Lechler T 2013. J Cell Biol. 213: 679 - 692. PubMed
  8. Shatirishvili M, et al. 2016. Cell Death Dis. 7:e2461. PubMed
  9. Madon-Simon M, et al. 2017. PLoS One. 12(6):e0180035. PubMed
  10. Lecland N, et al. 2019. Life Sci Alliance. 2:2. PubMed
  11. Miao Q, et al. 2019. Nat Commun. 10:4042. PubMed
  12. Suga H, et al. 2019. J Invest Dermatol. 139(9)1867. PubMed
Publication Library
RRID
AB_2565053 (BioLegend Cat. No. 905801)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Gene ID
2312 View all products for this Gene ID
UniProt
View information about Filaggrin on UniProt.org

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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