Direct-Blot™ HRP anti-Histone H3 Phospho (Ser28) Antibody

Pricing & Availability
Clone
HTA28 (See other available formats)
Other Names
Histone-H3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
HTA28_DB_HRP_Histone-H3-Phos_Antibody_121418.png
Western blot analysis of 15µg cell lysates from HeLa with or without 100 ng/mL Nocodazole treatment for 4 hours. Samples were resolved by electrophoresis (14% Tris Glycine gel), transferred to nitrocellulose, probed with 0.25 µg/mL Direct-Blot™ anti-Histone H3-Phosphorylated (Ser28) Antibody, clone HTA28. Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) Antibody (Cat. No. 819404) was used as the loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • HTA28_DB_HRP_Histone-H3-Phos_Antibody_121418.png
    Western blot analysis of 15µg cell lysates from HeLa with or without 100 ng/mL Nocodazole treatment for 4 hours. Samples were resolved by electrophoresis (14% Tris Glycine gel), transferred to nitrocellulose, probed with 0.25 µg/mL Direct-Blot™ anti-Histone H3-Phosphorylated (Ser28) Antibody, clone HTA28. Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) Antibody (Cat. No. 819404) was used as the loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Cat # Size Price Quantity Avail. Save
641017 25 µl £57
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641018 100 µl £195
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Description

H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide phosphorylated at serine 28 conjugated to KLH.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Hirata A, et al. 2004. J. Histochem. Cytochem. 52:1503.
  2. Goto H, et al. 1999. J. Biol. Chem. 274:25543.
  3. Ozawa K. 2008. Cytometry A 73:517.
  4. Goode NJ, et al. 2014. PLoS Genet. 10:1004323. PubMed
RRID
AB_2783362 (BioLegend Cat. No. 641017)
AB_2783363 (BioLegend Cat. No. 641018)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.

Regulation
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID
8290 View all products for this Gene ID
UniProt
View information about Phosphorylated Histone H3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/13/2018

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