Direct-Blot™ HRP anti-HDAC2 Antibody

Pricing & Availability
Clone
13G8C67 (See other available formats)
Regulatory Status
RUO
Other Names
Histone Deacetylase 2, YY1-Associated Factor 1, HD2, Transcriptional Regulator Homolog RPD3, YAF1
Isotype
Mouse IgG1, κ
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Product Citations
publications
13G8C67_DB-HRP_HDAC2_Antibody_WB_042017
Total cell lysate (15µg protein) from HepG2 cells was resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-HDAC2 or 1:5000, 1:20000 and 1:50000 Direct-Blot™ HRP anti-HDAC2 Antibody (upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
  • 13G8C67_DB-HRP_HDAC2_Antibody_WB_042017
    Total cell lysate (15µg protein) from HepG2 cells was resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-HDAC2 or 1:5000, 1:20000 and 1:50000 Direct-Blot™ HRP anti-HDAC2 Antibody (upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
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680105 100 µL £233
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Description

HDAC2 is a member of the class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups from the side chain of lysine residues on the N-terminal region of core histones. HDAC2 lacks DNA binding activity and executes its function by recruiting transcription factors and forming large transcriptional repressor complexes. In addition to histones, HDAC2 can deacetylate transcription factors and modify their transcriptional activity. HDAC2 is highly homologous to HDAC1 in regulating cell proliferation, differentiation, and apoptosis. HDAC2 has been shown to negatively regulate synaptic plasticity and memory formation. Elevated activity of HDAC2 has been associated with the loss of memory and neuronal degeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human HDAC2 peptide (471-488 a.a.) conjugated to KLH.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:5000-1:50000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2687039 (BioLegend Cat. No. 680105)

Antigen Details

Structure
488 amino acids with a predicted molecular weight of 55.4 kD. It contains a histone deacetylase domain.
Distribution

Nucleus.

Function
HDAC2 is a histone deacetylase responsible for the deacetylation of ε-amino acid group of lysine residues on the N-terminal tail of core histones. Forms of complex with transcription factors and acts as a transcriptional co-repressor.
Interaction
Forms histone deacetylase complexes with HDAC1, RBBP4, and RBBP7. Interacts with GFI1, SNW1, HDAC7, PRDM6, SAP30, SETDB1, SUV39H1, H2AFY, ATR, CBFA2T3, BCL-6, DNMT1, MINT, HDAC10, HCFC1, NRIP1, KDM4A, and PELP1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References

1. Winter M, et al. 2013. EMBO J. 32:3176.
2. Ma P, et al. 2013. PLoS Genet. 9:e1003377.
3. Kurita M, et al. 2012. Nat. Neurosci. 15:1245.
4. Segre CV, et al. 2011. J. Biomed. Biotechnol. 2011:690848.
5. Brunmeir R, et al. 2009. Int. J. Dev. Biol. 53:275.
6. Guan JS, et al. 2009. Nature 459:55.

Gene ID
3066 View all products for this Gene ID
UniProt
View information about HDAC2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All HDAC2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-HDAC2 13G8C67 WB,IP,ICC,KO/KD-WB,ChIP
Direct-Blot™ HRP anti-HDAC2 13G8C67 WB
Go To Top Version: 0    Revision Date: 04/21/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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