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Direct-Blot™ HRP anti-6-His Epitope Tag Antibody

Pricing & Availability
Clone
6-His (See other available formats)
Regulatory Status
RUO
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051118
Western blot analysis of 15 µg cell lysates from HeLa cells transfected with (+) or without (−) His-tagged plasmid. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.05, 0.1 and 0.5 µ/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-His-HRP antibody, clone 6-His. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
  • 6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051118
    Western blot analysis of 15 µg cell lysates from HeLa cells transfected with (+) or without (−) His-tagged plasmid. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.05, 0.1 and 0.5 µ/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-His-HRP antibody, clone 6-His. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
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906109 25 µL £77
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906110 100 µL £233
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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Purity
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 5.0 µg per ml (1:1000-1:10000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References

(PubMed link indicates BioLegend citation)
  1. Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed
  3. Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (ICC)
  5. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
  6. Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
  7. Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
Product Citations
  1. Chandrasekar SS, et al. 2021. Vaccines (Basel). 9:. PubMed
RRID
AB_2734681 (BioLegend Cat. No. 906109)
AB_2734682 (BioLegend Cat. No. 906110)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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