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Cyto-Last™ Buffer

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Regulatory Status
RUO
Other Names
CytoLast Buffer
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CytoLastBuffer_MQ1-17H12_PE1_070312
PMA+ionomycin-stimulated human peripheral blood lymphocytes (for 6 hours in the presence of monensin) were surface stained with CD3 APC and fixed. Cells were then permeabilized and intracellularly stained with human IL-2 (clone MQ1-17H12) PE at day 0 (top), or at day 14 (bottom) after storage in BioLegend's Cyto-Last™ Buffer.
  • CytoLastBuffer_MQ1-17H12_PE1_070312
    PMA+ionomycin-stimulated human peripheral blood lymphocytes (for 6 hours in the presence of monensin) were surface stained with CD3 APC and fixed. Cells were then permeabilized and intracellularly stained with human IL-2 (clone MQ1-17H12) PE at day 0 (top), or at day 14 (bottom) after storage in BioLegend's Cyto-Last™ Buffer.
  • CytoLastBuffer_MQ1-17H12_PE2_070312
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422501 100 mL £53
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Description

Cyto-Last™ Buffer is specially formulated for the storage of cytokine producing cells. When used, staining of intracellular and/or extracellular targets for flow cytometric analysis can be delayed for up to two weeks. Cells should be stored at 4°C during this time. This unique buffer ensures cells maintain a background staining signal equal to that of freshly prepared cells, while also retaining high specific immunofluorescent staining against target antigens.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store Cyto-Last™ Buffer between 2°C and 8°C.
Application

ICFC - Quality tested
Application Notes

Staining Procedure:
1. Prepare target cells of interest and perform surface staining as described in BioLegend's Cell Surface Immunofluorescence Staining Protocol. (Note: staining with tandem-dye-conjugated antibodies (e.g., PE/Cy7, APC/Cy7, etc.) is not recommended as color compensation shifts may occur with long-term storage.)
2. Fix the cells with 0.5 mL/tube BioLegend's Fixation Buffer (Cat. No. 420801) at room temperature, in the dark, for 20 minutes.
3. Centrifuge at 350 x g for 5 minutes; discard supernatant.
4. Resuspend the cells in 0.5-1 mL/tube Cyto-Last™ Buffer, mix, cap the tubes, and then store at 4°C in the dark (Note: Cyto-Last™ Buffer can also be used for preserving cells in bulk at a cell concentration of 0.5-2.0 x 106 cells/mL.).
5. Take out the tubes at desired time points. Remove Cyto-Last™ Buffer by centrifugation, permeabilize the cells with BioLegend's Permeabilization Wash Buffer (Cat. No. 421002), and perform intracellular cytokine staining.

Antigen Details

Gene ID
NA

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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