- Other Names
- Fc Receptor Blocking Solution
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Human Fc receptors (FcRs) are molecules that regulate the immune responses. FcRs are expressed by several hematopoietic cells. Due to FcRs-mediated Ig Fc binding, false positive or false negative results of immunofluorescent staining could occur. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP is specially formulated to block the FcR-involved unwanted staining without interfering with the antibody-mediated specific staining of human cells. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP contains ultra-low endotoxin levels (<0.01EU/µg of protein) and is tested negative for mycoplasma and microbial growths.
This GMP product is for research and further ex vivo bioprocessing use only.
- Serum-Free, Defined, containing no preservatives. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Storage & Handling
- Store between 2°- 8°C. Handle under aseptic conditions.
FC - Quality tested
- Recommended Usage
5 μl of Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP per million cells in 100 μl staining buffer.
- Application Notes
This buffer contains specialized human IgG. It is not recommended to be used for staining human IgG. Handle as biohazard agent. Add 5 μl of Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP per million cells in 100 μl staining buffer, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.