Brilliant Violet 785™ anti-human CD337 (NKp30) Antibody

Pricing & Availability
Clone
P30-15 (See other available formats)
Other Names
NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P30-15_BV785_CD337_Antibody_060319
Human peripheral blood lymphocytes were stained with CD56 FITC and CD337 (NKp30) (clone P30-15) Brilliant Violet 785™ (left) or mouse IgG1, κ Brilliant Violet 785™ isotype control (right).
  • P30-15_BV785_CD337_Antibody_060319
    Human peripheral blood lymphocytes were stained with CD56 FITC and CD337 (NKp30) (clone P30-15) Brilliant Violet 785™ (left) or mouse IgG1, κ Brilliant Violet 785™ isotype control (right).
See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Avail. Save
325229 25 tests £124
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325230 100 tests £262
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Description

The p30-15 monoclonal antibody recognizes CD337 also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3. NKp30 is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp30 has an apparent molecular weight of 30 kD and six isoforms are produced by alternative splicing. NKp30 is expressed on resting and activated NK cells. NKp30 enhances NK cell cytolysis of tumor cellts that are deficient in MHC class I molecules. NKp30 has been shown to associate with CD59 and TCRζ. The p30-15 antibody against human NKp30 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human NKp30
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 785™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution1,3,5. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).

Application References

(PubMed link indicates BioLegend citation)
  1. Stark S, et al. 2005. J. Immunol. Methods 296:149. (Block)
  2. Mansater I, et al. 2008. J. Immunol. 181:1869. PubMed
  3. Markel G, et al. 2009. PLOS One 4:e5597. (Block)
  4. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  5. Byrd A, et al. 2007. PLoS One. 2:e1339. (Block)

Antigen Details

Structure
Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Six isoforms produced by alternative splicing.
Distribution

IL-2 activated NK cells and NK cell lines, some γ/δ T cells activated in vitro

Function
NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction
CD59, TCRζ
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Pende D, et al. 1999. J. Exp. Med. 190:1505.

Gene ID
259197 View all products for this Gene ID
UniProt
View information about CD337 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/03/2019

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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