Brilliant Violet 785™ anti-human CD335 (NKp46) Antibody

Pricing & Availability
Clone
9E2 (See other available formats)
Other Names
NKp46, NCR1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9E2_BV785_CD335_Antibody_060319.png
Human peripheral blood lymphocytes were stained with CD56 PE and CD335 (NKp46) (clone 9E2) Brilliant Violet 785™ (left) or Mouse IgG1, κ Brilliant Violet 785™ isotype control (right)
  • 9E2_BV785_CD335_Antibody_060319.png
    Human peripheral blood lymphocytes were stained with CD56 PE and CD335 (NKp46) (clone 9E2) Brilliant Violet 785™ (left) or Mouse IgG1, κ Brilliant Violet 785™ isotype control (right)
See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Avail. Save
331945 25 tests £144
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331946 100 tests £276
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Description

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NKp46-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 785™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 9E2 has been shown to block NK activation through NKp46.6

Application References

(PubMed link indicates BioLegend citation)
  1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
  2. Kalberer CP, et al. 2003. Blood 102:127.
  3. Chen Y, et al. 2007. J. Immunol. 179:2766.
  4. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Achdout H. et al. 2010. J. Virol. 84:3993.

Antigen Details

Structure
Type I membrane glycoprotein (46 kD)
Distribution

Expressed on resting and activated NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.

Gene ID
9437 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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